.alpha.-amylase gene

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435 691, 435 712, 4352402, 4352523, 43525233, 43525231, 4353201, 536 232, 536 231, 935 14, 935 29, 935 56, 935 72, 935 73, 935 74, C12N 1556, C12N 1563, C12N 1575, C12N 121

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active

053668839

ABSTRACT:
The present invention relates, in general, to a cloned .alpha.-amylase gene, and, in particular, to a cloned hyperthermophilic .alpha.-amylase gene and to methods of producing .alpha.-amylase using same.

REFERENCES:
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A. Belyavsky et al. "PCR-based cDNA library construction . . . " Nuc. Acids. Res. 17(8) 2919-2932 (Apr. 1989).
S. Fukusumi et al. "Cloning and Nucleotide Sequence of a heat . . . " Eur. J. Biochem. 174:15-21 (May 1988).
R. Koch et al. "Extremely thermostable amylolyhi enzyme . . . " FEMS Microbiol. Lett. 71:21-26 (Sep. 1990).
S. L. Berger et al. (eds.) "Guide to Molecular Cloning Techniques." Meth. in Enzymol. vol. 132:393-399, 415-423, 432-447, 661-704 (1987).
M. P. Deutscher (ed.) "Guide to Protein Purification" Meth. in Enzymol. vol. 182:602-613, 738-751 (1990).
Koch, "Purification and properties of a hyperthermoactive .alpha.-amylase from the archaebacterium Pyrococcus woesei", Arch. Microbiol. (1991) 155:572-578.
Brown et al, "Characerization of Amylolytic Enzyme Activities Associated with the Hyperthermophilic Archaebacterium Pyrococcus furiosus", Applied and Environmental Microbiology, Jul. 1990, pp. 1985-1991.

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