.alpha.-amylase assay using modified oligosaccharide and process

Details .alpha.-amylase assay using modified oligosaccharide and process .alpha.-amylase assay using modified oligosaccharide and process

1992-11-06

1994-06-07

Griffin, Ronald W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 171, 435 22, C12Q 140, C07H 504, C07H 1500

Patent

active

053190785

ABSTRACT:
.alpha.-Amylase activity can be determined rapidly and precisely by using as a substrate a modified oligo-saccharide wherein a benzylozymethyl group or a halo-methyl group is bonded to the C.sub.6 position of non-reducing-end glucose residue and the reducing-end glucose residue is bonded to a group which exhibits an optically measurable change upon cleavage. Processes for producing such a compound are also disclosed.

REFERENCES:
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patent: 4622295 (1986-11-01), Ikenaka
patent: 4649103 (1987-03-01), Blair
patent: 4681841 (1987-07-01), Matsumoto
patent: 5192666 (1993-03-01), Ikenaka et al.
Omichi et al. J. Biochem., 93, 1055 (1983).
Omichi et al., J. Biochem., 99, 1245 (1986).
Methods in Carbohydrate Chemistry, vol. 2, p. 236 (1963).

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