.alpha.-amylase assay using modified oligosaccharide and process

Details .alpha.-amylase assay using modified oligosaccharide and process .alpha.-amylase assay using modified oligosaccharide and process

1989-12-29

1993-03-09

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 18, 435201, 435 14, 435 4, 536 186, 536 174, 536 179, 536 171, 536 184, 536 179, 536 187, 536 46, 536 50, 536123, C12Q 140, C12Q 134, C12Q 154, C12Q 100, C07H 100, C07H 300, C07H 1500, C07H 1700, C07H 1702, C08B 3700, C08B 3018, C07G 300

Patent

active

051926660

ABSTRACT:
.alpha.-Amylase activity can be determined rapidly and precisely by using as a substrate a modified oligosaccharide wherein a benzyloxymethyl group or a halomethyl group is bonded to the C.sub.6 position of non-reducing-end glucose residue and the reducing-end glucose residue is bonded to a group which exhibits an optically measurable change upon cleavage. Processes for producing such a compound are also disclosed.

REFERENCES:
patent: 4000042 (1976-12-01), Adams
patent: 4622295 (1986-11-01), Ikenaka et al.
patent: 4649108 (1987-03-01), Blair
patent: 4681841 (1987-07-01), Matsunoto et al.
Ohmichi et al., Chemical Abstracts, vol. 103, No. 1, Jul. 8, 1989, p. 256, Abstract 2743p.

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