Alpha-amylase assay and substrates for use therein

Sugar – starch – and carbohydrates – Products – Miscellaneous

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435 22, 435 14, 435 15, 435 25, 435 26, 435 97, 435 95, 127 71, C12Q 140, C12Q 126, C12P 1918, C13F 300

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045057567

ABSTRACT:
A maltodextrin phosphorylase limit dextrin in the presence of maltodextrin phosphorylase and inorganic phosphate, is used as a substrate for alpha-amylase, which initiates a series of enzymatic reactions resulting in a chromogen response which can be used to measure the concentration of alpha-amylase in a body fluid. A novel limit dextrin and its preparation also are described.

REFERENCES:
patent: 2891869 (1959-06-01), Langlors
Kruger et al., The Use of Reduced beta-limit Dextrin as Substrate in an Automated Amylase Assay, Cereal Chem., V149, (1972), pp. 453-458.
Kerr, Chemistry and Industry of Starch, 2nd ed., (1950), Academic Press, Inc., N.Y., pp. 210, 212-214, 414.
Barman, Enzyme Handbook, Springer-Verlag, N.Y., (1969), pp. 302, 303, 562-564.
Kruger, "Changes in the Amylases of Hard Spring Wheat during Growth and Maturation", Ceral Chem., vol. 49, No. 4, (1972a), pp. 379-390.
Chao et al., Kinetic Mechanism of Maltodextrin Phosphorylase, Biochemistry, vol. 8, 1969, 1459-1466.
Radley, Starch and its Derivatives, Chapman and Hall Ltd., London, 1968, pp. 482-483.

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