Alpha amylase assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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435 8, 435 14, 435 18, C12Q 140, C12Q 166, C12Q 154, C12Q 134

Patent

active

046491082

ABSTRACT:
Method of measuring the amount of .alpha.-amylase in a liquid sample, including the steps of providing an oligosaccharide substrate for .alpha.-amylase, the substrate being characterized in that it contains at least 3 glucose units, its reducing-end glucose unit is bonded, via a bond cleavable by .alpha.- or .beta.-glucosidase, to a label which exhibits an optically measurable change upon cleavage of the bond, and its terminal glucose unit is bonded to a blocking substituent which inhibits cleavage by exo-enzymes of the bond between the terminal glucose unit and the adjacent glucose unit; contacting the sample with the oligosaccharide substrate and with a first exo-enzyme capable of cleaving the bond between the reducing-end glucose unit and the label, and measuring the optically measurable change as a measure of .alpha.-amylase in the sample.

REFERENCES:
patent: 3876375 (1975-04-01), Maurukas
patent: 4102747 (1978-07-01), Driscoll et al.
patent: 4145527 (1979-03-01), Burns et al.
patent: 4147860 (1979-04-01), Farnham et al.
patent: 4153511 (1979-05-01), Modrovich
patent: 4233403 (1980-11-01), Menson et al.
patent: 4250254 (1981-02-01), Modrovich
patent: 4271264 (1981-06-01), Modrovich
patent: 4277562 (1981-07-01), Modrovich
patent: 4282316 (1981-08-01), Modrovich
patent: 4310625 (1982-01-01), Modrovich
patent: 4550077 (1985-10-01), Woodbridge et al.
M. Matsui et al, "Research of Reactivity and Hydrolysis Type on Various Substrates for .alpha.-Amylase Assay by H.P.L.C.; Japan Clinical Chemistry Association Summer Seminar; Jul. 1982.
Marshall et al. (1977) New Serum alpha-Amylase Assay of High Sensitivity, Chimica Acta 76, 277-283.
Marshall, Analytical Biochemistry (1978) 85, 541-549.
Calbiochem-Behring (1981) Pantrak E.K. Amylase.
Biomedix (1980) Amylase (U.V.) DeltaTest Assay.

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