Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1984-07-26
1987-03-10
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 8, 435 14, 435 18, C12Q 140, C12Q 166, C12Q 154, C12Q 134
Patent
active
046491082
ABSTRACT:
Method of measuring the amount of .alpha.-amylase in a liquid sample, including the steps of providing an oligosaccharide substrate for .alpha.-amylase, the substrate being characterized in that it contains at least 3 glucose units, its reducing-end glucose unit is bonded, via a bond cleavable by .alpha.- or .beta.-glucosidase, to a label which exhibits an optically measurable change upon cleavage of the bond, and its terminal glucose unit is bonded to a blocking substituent which inhibits cleavage by exo-enzymes of the bond between the terminal glucose unit and the adjacent glucose unit; contacting the sample with the oligosaccharide substrate and with a first exo-enzyme capable of cleaving the bond between the reducing-end glucose unit and the label, and measuring the optically measurable change as a measure of .alpha.-amylase in the sample.
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M. Matsui et al, "Research of Reactivity and Hydrolysis Type on Various Substrates for .alpha.-Amylase Assay by H.P.L.C.; Japan Clinical Chemistry Association Summer Seminar; Jul. 1982.
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Calbiochem-Behring (1981) Pantrak E.K. Amylase.
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Genzyme Corporation
Warden Robert J.
White Patricia Kate
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