Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1984-09-27
1987-11-24
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 69, 435 70, 435228, 4351723, 935 11, 935 51, C12P 2100, C12P 2106, C12P 2102, C12N 980, C12N 1500
Patent
active
047089342
ABSTRACT:
Peptidyl-glycine .alpha.-amidating monooxygenase is an enzyme extractable from medullary thyroid carcinoma cell lines and tissue samples, having a molecular mass of about 60,000 to 65,000 daltons. It has been purified so as to exhibit a single, homogeneous, well-defined band using electrophoretic procedures performed on SDS-polyacrylamide gels, and has a specific enzymatic activity of at least 50 mU per mg protein. The free or immobilized enzyme, in the presence of Cu.sup.+2 ions, ascorbate, and oxygen, can be used to prepare an .alpha.-amidated protein from a polypeptide substrate possessing a carboxyl-terminal glycine residue. The purified enzyme can be used as an antigen in order to produce enzyme-specific monoclonal antibodies, and can provide the information necessary to design and construct prokaryotes or other appropriate unicellular organisms or host cells isolated from multicellular organisms which possess peptidyl-glycine .alpha.-amidating capability.
REFERENCES:
Eipper et al, Proc. Natl. Acad Sci, vol. 80, pp. 5144-5148, 1983.
Eipper et al, Peptides, vol. 4, pp. 921-928, 1983.
Bradbury et al., Nature, vol. 298, pp. 686-688, 1982.
Hsueh et al, in Proteases, Potential Role in Health and Disease, Plenum Press, New York and London, (Horl & Heidland, eds.) pp. 141-151, 1984.
Amara et al, Proc. Natl. Acad Scivol. 77, pp. 4444-4448, 1980.
Pharmacia Fine Chemicals Catalogue, 1984, p. 9.
Glembotski et al, J. Biol. Chem, vol. 259(10), pp. 6385-6392, 1984.
Chem Abst., p. 244, 101:50587w.
Mains et al, Endocrinology, vol. 116, pp. 2505-2515, 1985.
Eipper et al, Endocrinology, vol. 116, pp. 2497-2504, 1985.
Gilligan James P.
Jones Barry N.
Huleatt Jayme A.
Unigene Laboratories Inc.
Wiseman Thomas G.
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