.alpha.-1-antichymotrypsin, analogues and methods of production

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

530350, 530397, 930250, C07H 2104, C07K 1300, C12N 1522

Patent

active

053670647

ABSTRACT:
The invention provides .alpha.-1-antichymotrypsin and protein preparations comprising human .alpha.-1-antichymotrypsin produced by E. coli cells transformed with a DNA sequence encoding human .alpha.-1-antichymotrypsin. The invention also provides methods for producing .alpha.-1-antichymotrypsin. The invention further provides analogues of .alpha.-1-antichymotrypsin that exhibit antichymotrypsin, anti-trypsin and anti-thrombin activity and methods of producing the analogues.

REFERENCES:
patent: 4732973 (1988-03-01), Barr et al.
patent: 5079336 (1992-01-01), Rubin
Studier, et al., "Use of Bacteriophage T7 RNA Polymerase to Direct Selective High-level Expression of Cloned Genes," J. Mol. Biol. 189:113-130 (1986).
Tsuda, et al., "Purification Properties and Identification of a Serum DNA Binding Protein (64DP) and Its Microheterogeneity," Tokai J. Exp. Clin. Med. 7:201-211 (1982).
Hill, et al., "Plasma protease inhibitors in mouse and man: divergence within the reactive centre regions," Nature 311:175-177 (1984).
Young, et al., "Efficient isolation of genes by using antibody probes," Proc. Natl. Acad. Sci., USA 80:1194-1198 (1983).
Chandra, et al., "Sequence Homology Between Human .alpha.1-Antichymotrypsin, .alpha.1-Antitrypsin, and Antithrombin III,"Biochemistry 22:5055-5060 (1983).
DelMar, et al., "A Sensitive New Substrate For Chymotrypsin," Anal. Biochem. 99:316-320 (1979).
Ardelt, et al., "Turkey Ovomucoid Third Domain Inhibits Eight Different Serine Proteinases of Varied Specificity on the Same . . . Leu.sup.18 -Glu.sup.19 . . . Reactive Site," Biochemistry 24:5313-5320. (1985).
Laemmli, U. K. "Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4," Nature (London) 227:680-685 (1970).
Beatty, et al., "Kinetics of Association of Serine Proteinases with Native and Oxidized .alpha.-1-Proteinase Inhibitor and .alpha.-1-Antichymotrypsin," J. Biol. Chem. 225:3931-3934 (1980).
Towbin, et al., "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications," Proc. Nat'l Acad. Sci USA 76:4350-4354 (1979).
Carrell and Owen, "Plakalbumin, .alpha..sub.1 -antitrypsin, anithrombin and the mechanism of inflammatory thrombosis," Nature 317:730-732 (1985).
Wachter, et al., "Cloning A Serine Protease Inhibitor Gene With An Antibody Specific Probe", Abstract, Jan. 1987, American Academy of Allergy and Immunology.
Wachter, et al., "Cloning And Antigenic Determinants Of Serine Protease Inhibitors", Abstract, Jan. 1987, Annals of Allergy.
Maniatis, et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp. 249-255 (1982).
Sambrook, et al., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp. 1.74-1.84 (1989).
Morii and Travis, "Amino Acid Sequence at the Reactive Site of Human .alpha..sub.1 -Antichymotrypsin," J. Biol. Chem. 258:12749-12752 (1983).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

.alpha.-1-antichymotrypsin, analogues and methods of production does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with .alpha.-1-antichymotrypsin, analogues and methods of production, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and .alpha.-1-antichymotrypsin, analogues and methods of production will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1992077

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.