.alpha.-1,3/4-fucosidase gene

Details .alpha.-1,3/4-fucosidase gene .alpha.-1,3/4-fucosidase gene

1995-05-17

1997-06-10

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4352523, 4353201, 536 237, C12N 926, C12N 121, C12N 1563, C07H 2104

Patent

active

056374909

ABSTRACT:
An isolated .alpha.-1,3/4-fucosidase gene is provided which codes for an .alpha.-1,3/4-fucosidase enzyme. The isolated gene codes for the fucosidase enzyme having the amino acid sequence shown in SEQ ID No. 1. The gene may have the DNA sequence shown in SEQ ID No. 2. A process for producing the fucosidase enzyme is also provided, which comprises cultivating a transformant into which a recombinant plasmid containing the .alpha.-1,3/4-fucosidase gene according to the invention has been introduced to produce the fucosidase enzyme encoded thereby, and harvesting the fucosidase enzyme from the culture.

REFERENCES:
Fisher, K.J. et al. (1989) "Isolation and sequence analysis of a cDNA encoding rat liver alpha-fucosidase" Biochem. J. 264:695-701 1989.
Alberts, B. et al. Molecular Biology of the Cell, Second Edition. New York: Garland Publ. Inc. 1989, pp. 265-266 1989.
Fukushima, H. et al. (1985) "Molecular cloning of a cDNA for human alpha-L-fucosidase" Proc. Nat'l. Acad. Sci., USA. 82:1262-1265 Feb. 1985.
Muller-Taubenberger, A. et al. (1989) "A developmentally regulated gene product from Dictyostelium discoideum shows high homology to human alpha-L-fucosidase" FEBS Lett. 246(1,2):185-192 Mar. 1989.
Sano et al., "Purification and Characterization of .alpha.-L-Fucosidase from Strepomyces Species", The Journal of Biological Chemistry, vol. 267, No. 3, pp. 1522-1527, Jan. 25, 1992.
Midor Ogata-Arakawa et al., ".alpha.-L-Fucoisidases from Almond Emulsin: Characterization of the Two Enzymes with Different Specificities", Archives of Biochemistry and Biophysics 181, pp. 353-358, (1977).
Michael J. Imber et al., "Purification of an Almond Emulsin Fucosidase on Cibacron Blue-Sepharose and Demonstration of Its Activity Toward Fucose-Containing Glycoproteins", The Journal of Biological Chemistry vol. 257, No. 14, Issue of Jul. 25, 1982 pp. 8205-8210.
Peter Scudder et al., "The Isolation by Ligand Affinity Chromatography of a Novel Form of .alpha.-L-Fucosidase from Almond", The Journal of Biological Chemistry, vol. 265, No. 27, Issue of Sep. 25, 1990, pp. 16472-16477.
Mutsumi Sano et al., "Purification and Characterization of .alpha.-L-Fucosidase From Streptomyces Species", vol. 267, No. 3, Issue of Jan. 25, 1992, pp. 1522-1527.

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