Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-09-29
1996-07-09
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 79, 435 794, 435962, 435967, 436518, 436531, 436536, G01N 33536, G01N 33543
Patent
active
055344127
ABSTRACT:
Methods and compositions are provided for the determination of cross-reactive alleles, where there is no antibody which will specifically distinguish between the two alleles. Particularly, the method employs an antibody which binds to the two alleles bound to a surface, an antibody specific for one of the alleles, a labeled conjugate which binds to a consensus sequence present in both alleles and positive and negative controls. By having an enhanced value where the interfering allele is present as compared to a value for the target allele, one can distinguish between the various alternatives involving the presence or absence of one or both alleles.
REFERENCES:
patent: 5420013 (1995-05-01), Pouletty et al.
Albrecht, J. and Muller, H. A. G., Clin. Chem, (1987), 33:1618-1623. HLA-B27 Typing by Use of Flow Cytofluorometry.
Thorsby, E., et al., Tissue Antigens, (1983), 21:148-158. HLA-D Restriction of Antigen-Specific Proliferative T Cell Responses.
Sakaguchi, K., et al., Human Immunology, (1988), 21:193-207. Anti-HLA-B7, B27, Bw42, Application for a Double Beerminant Immunoassay.
Ferreira, A., et al., Clin. Chem. Acta, (1988), 174:207-212. Quantification of Soluble Serum HLA Class l Antigens in Healthy Volunteers and AIDS Patients.
Davies, H. FF. S., et al., Transplantation, (1989), 47:524-527. Soluble HLA Antigens in the Circulation of Liver Graft Recipients.
Hill, A. V. S., et al., Lancet, (1991), 337:640-642. HLA Class l Typing by PCR:HLA-B27 and an African B27 Subtype.
Doxiadis, I. and Grosse-Wilde, H., Vox Sang, (1989), 56:196-199. Typing for HLA Class l Gene Products Using Plasma as Source.
Hansen, T. and Hannestad, K., Tissue Antigens, (1987), 30:198-203. Simple Rosette Assay for HLA-B27 Typing of Whole Blood Samples.
Thurau, S. R., et al., Tissue Antigens, (1989), 33:511-519. Expression and Immunogenicity of HLA-B27 in High Transfection Recipient P815:a New Method to Induce Monoclonal Antibodies Directed Against HLA-B27.
Villar, L. M., et al., Eur. J. Immunol., (1989), 19:1835-1839. Detection of Soluble Class l Molecules (non HLA-A or HLA-B) in Serum, Spleen Membranes and Lymphocytes in culture.
Wu, D. Y., et al., DNA, (1989), 8:135-142. Direct Analysis of Single Nucleotide Variation in Human Dna and Rna Using in Situ Dot Hybridication.
Trapani, J. A., et al., Immunol. Cell Biol. (1988), 66:215-219. Immunoradiometric Assay for the Rapid Detection of HLA-B27.
Chang Chin-Hai
Pouletty Philippe
Rowland Bertram I.
SangStat Medical Corporation
Saunders David
Sherwood Pamela J.
LandOfFree
Alloantigen enhancement assay does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Alloantigen enhancement assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Alloantigen enhancement assay will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1866579