Allergenic latex protein

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues

Reexamination Certificate

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Details

C530S350000

Reexamination Certificate

active

07732566

ABSTRACT:
The present invention relates to a protein found in natural rubber that can induce an allergic reaction in persons who have been sensitised to it. The invention provides for the process of isolating and purifying the protein and describes the characteristics of the protein, including its molecular weight, isoelectric point, amino acid sequence and allergenicity. The invention also describes the isolation and cloning a the DNA that encodes the protein. The production of the recombinant version of the protein using a protein expression vector is described.

REFERENCES:
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Arif et al. ‘Isolation and characterization of the Early Nodule-specific Proteing homologue (Hev b 13) and Allergenic lipolytic esterase fromHevea brasiliensislatex.’ J. Biol. Chem. 279(23):23933-23941, 2004.
Beezhold et al., “Identification of Latex Protein Allergens”, Paper presented at ‘International Conference on Latex Protein Allergy: The Latest Position’, 1995, pp. 19-27.
Sowka et al., “cDNA cloning of the 43-kDa latex allergen Hev b 7 with sequence similarity to patatins and its expression in the yeastPichia pastoris”, Eur. J. Biochem, vol. 255 (1998), pp. 213-219.
Kostyal et al., “Cloning and characterization of a latex allergen (Hev b 7): homology to patatin, a plant PLA2”, Clin. Exp. Immunol. 1998, vol. 112, pp. 355-362.
Yeang et al., “Allergenic proteins of natural rubber latex”, Academic Press, Methods 27 (2002), pp. 32-45.
Lazar et al., “Transforming Growth Factor α: Mutation of Aspartic Acid 47 and Leucine 48 Results in Different Biological Activities”, Molecular and Cellular Biology, vol. 8 (3), 1988, pp. 1247-1252.
Bowie et al., “Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions”, Science, vol. 247, pp. 1306-1310.
Wang et al., “A Single Amino Acid Determines Lysophospholipid Specificity of the S1P1(EDG1) and LPA1(EDG2) Phospholipid Growth Factor Receptors”, Journal of Biological Chemistry, vol. 276 (52), 2001, pp. 49213-49220.
Burgess et al., “Possible Dissociation of the Heparin-binding and Mitogenic Activities of Heparin-binding (Acidic Fibroblast) Growth Factor-1 from Its Receptor-binding Activities by Site-directed Mutagenesis of a Single Lysine Residue”, Journal of Cell Biology, vol. 111, 1990, pp. 2129-2138.

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