Allergen detection

Chemistry: analytical and immunological testing – Peptide – protein or amino acid

Reexamination Certificate

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Details

C436S164000, C436S166000, C436S169000, C436S805000, C436S808000, C435S023000

Reexamination Certificate

active

10009109

ABSTRACT:
A method of determining allergen activity in dust comprises: providing a dust sample; extracting from the dust sample at least one breakdown component or proteins or peptides; reacting the extracted at least one breakdown component with a colorimetric amine detection reagent such as TNBSA; and quantitatively measuring the intensity of any resulting coloration. The allergen activity may be gauged by the intensity of coloration.

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Cayot, P. and Tainturier, G., “The Quantification of Protein Amino Groups by the Trinitrobenzene-sulfonic Acid Method: A Reexamination,” Analytical Biochemistry, vol. 249, pp. 184-200 (1997).

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