Alleles of the PRPD1 gene from coryneform bacteria

Details Alleles of the PRPD1 gene from coryneform bacteria Alleles of the PRPD1 gene from coryneform bacteria

2011-01-19

2011-12-20

Noakes, Suzanne M (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

C435S252300, C435S252320, C435S252800, C435S320100, C435S069100, C435S071100, C536S023100

Reexamination Certificate

active

08080395

ABSTRACT:
An isolated mutant of coryneform bacteria comprising a gene encodes a polypeptide having 2-methylcitrate dehydratase activity, where the polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids except L-proline is present at position 272 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having 2-methylcitrate dehydratase enzymic activity, which comprises at position 272 of the amino acid sequence or a corresponding or comparable position a proteinogenic amino acid except L-proline is described. A method for producing a recombinant coryneform bacterium and L-amino acids. A recombinant microorganism, L-Lysine-containing feed additive, and L-Tryptophan-containing feed additive is also described.

REFERENCES:
Claes et al. (Identification of Two prpDBC Gene Clusters in Corynebacterium glutamicum and Their Involvement in Propionate Degradation via the 2-Methylcitrate Cycle, Journal of Bacteriology, May 2002, p. 2728-2739 vol. 184, No. 10).

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