Alkylguanyltransferase assays

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S007500

Reexamination Certificate

active

11373623

ABSTRACT:
This invention provides a method to determine alkylguanyltransferase activity in a sample, comprising steps of placing the sample in an appropriate condition so that the AGT is functional; contacting the sample with an AGT Detector under conditions permitting the binding of AGT and AGTD to produce a signal; and measuring the signal, thereby determining the AGT activity in said sample. This invention provides different uses of this method.

REFERENCES:
patent: 2002/0076785 (2002-06-01), Glotzer et al.
patent: 2004/0115130 (2004-06-01), Johnsson et al.
Goodtzova et al. Activation of Human O6-Alkylguanine-DNA Alkyltransferase by DNA; Biochemistry, vol. 33, No. 28 (1994) pp. 8385-8390.
Noll et al. Covalent Capture of a Human O6-Alkylguanine Alkyltransferase-DNA Complex Using N1,O6-Ethanoxanthosine, a Mechanism-Based CrossLinker; Nucleic Acids Research, vol. 29, No. 19 (2001) pp. 4025-4034.
Ishibashi, et al., “Artificial Control of Nuclear Translocation of DNA Repair Methyltransferase”, J. Biol. Chem. vol. 269, pp. 7645-7650 (1994).
Ishibashi, et al., “Intercellular Localization and Function of DNA Repair Methyltransferase in Human Cells”, Mutat. Res. vol. 315, pp. 199-212(1994).
Citron, et al., “06-methylguanine-DNA Methyltransferase in Normal and Malignant Tissue of the Breast”, Cancer Investig. vol. 12, pp. 605-610 (1994).
Kokkinakis, et al., “Role of 06-Methylguanine-DNA Methyltransferase in the Resistance of Pancreatic Tumors to DNA Alkylating Agents”, Cancer Res. vol. 57, pp. 5360-5368 (1997).
Lage, et al., “Involvement of the DNA Mismatch Repair System in Antineoplastic Drug Resistance”, Cancer Res. Cln. Or. vol. 125, pp. 156-165(1999)
Chinnasamy, et al., “06-Benzylguanine Potentiates The In Vivo Toxicity and Clastogenicity of Temozolomide and BCNU in Mouse Bone Marrow”, Blood. vol. 89, pp. 1566-1573 (1997).
Belanich, et al., “Retrospective Study of the Correlation Between the DNA Repair Protein Alkytransferase and Survival of Brain Tumor Patients Treated with Carmustine”, Cancer Res. vol. 56, pp. 783-788 (1996).
Jaeckle, et al., “Correlation of Tumor 06 Methylguanine-DNA methyltransferase Levels With Survival of Malignant Astrocytoma Patients Treated With Bis-Chloroethylnitrosourea: A Southwest Oncology Group Study”, J. Clin. Ocol. vol. 16, pp. 3310-3315 (1998).
Wu, et al., “Measurement of 06-Alkylguanine-DNA Alkyltransferase Activity in Human Cells and Tumor Tissues by Restriction Endonuclease Inhibition”, Cancer Res. vol. 47, p. 6229 (1987).
Gerson, et al., “06 Alkylguanine-DNA Alkyltransferase Activity in Human Myeloid Cells”, J. Clin. Invest. vol. 76, p. 2106 (1985).
Kreklau, et al., “A Novel Fluorometric Oligonucleotide Assay to Measure o(6)-Methylguanine DNA Methyltransferase, Methylpurine DNA Glycosylase, 8-oxyguanine DNA Glycosylase and Abasic Endonuclease Activities: DNA Repair Status in Human Breast Carinoma Cells Overexpressing Methylpurine DNA Glycosylase”, J. Clin. Nucleic Acid Res., vol. 29, p. 2558 (2001).
Noll, et al., “Covalent Capture of a Human O(6)-Alkylguanine Alkyltransferase-DNA Complex Using N(1), O(6)-ethanoxanthosine, a Mechanism-Based Crosslinker”, J. Clin. Nucleic Acid Res., vol. 29, p. 4025 (2001).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Alkylguanyltransferase assays does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Alkylguanyltransferase assays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Alkylguanyltransferase assays will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3758184

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.