Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1994-11-30
1998-04-21
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435219, 435220, 4352525, C12N 950, C12N 954
Patent
active
057416931
DESCRIPTION:
BRIEF SUMMARY
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation of PCT/DK93/00218 filed Jul. 2, 1993, published as WO94/01532 Jan. 20, 1994, which is incorporated herein by reference.
TECHNICAL FIELD
The present invention relates to novel microorganisms, novel enzymes obtainable herefrom, and to a method of producing the novel enzymes. More specifically, the invention relates to novel enzymes obtainable from strains of the novel alkalophilic species Bacillus sp. AC13.
Moreover, the invention relates to a method for producing the enzymes of the invention, and to the use of the enzymes, particularly in detergents or in the paper pulp industry.
SUMMARY OF THE INVENTION
It is an object of the present invention to provide novel microorganisms capable of expressing valuable novel enzymes for industrial applications, as well as novel enzymes of different kinds obtainable from these organisms.
Accordingly, the invention provides isolated biologically pure cultures of strains of the alkalophilic species Bacillus sp. AC13.
In another aspect, the invention provides enzymes obtainable from strains of Bacillus sp. AC13, and having immunochemical properties identical or partially identical to those of an enzyme derived from Bacillus sp. AC13, NCIMB No. 40482.
In a more specific aspect, the invention provides proteases obtainable from strains of Bacillus sp. AC13, and having immunochemical properties identical or partially identical to those of a protease derived from Bacillus sp. AC13, NCIMB No. 40482.
In another specific aspect, the invention provides xylanases obtainable from strains of Bacillus sp. AC13, and having immunochemical properties identical or partially identical to those of a xylanase derived from Bacillus sp. AC13, NCIMB No. 40482.
In a third specific aspect, the invention provides cellulases obtainable from strains of Bacillus sp. AC13, and having immunochemical properties identical or partially identical to those of a cellulase derived from Bacillus sp. AC13, NCIMB No. 40482.
In a third aspect, the invention provides a process for the preparation of an enzyme of the invention, the process comprising cultivation of a strain of Bacillus sp. AC13, preferably the strain Bacillus sp. AC13, NCIMB No. 40482, or a mutant or a variant thereof, in a suitable nutrient medium, containing carbon and nitrogen sources and inorganic salts, followed by recovery of the desired enzyme.
In further aspects, the invention provides detergent additives and detergent compositions comprising an enzyme of the invention.
Moreover, the invention relates to the use of a xylanase of the invention in processes for treatment of lignocellulosic pulp.
BRIEF DESCRIPTION OF DRAWINGS
The present invention is further illustrated by reference to the accompanying drawings, in which:
FIG. 1 shows the relative activity (% rel.) of a protease of the invention at various pH, determined at 25.degree. C. with casein as substrate; and
FIG. 2 shows the relative activity (% rel.) of a protease of the invention at various temperatures (.box-solid.Buffer pH 9.5; .quadrature. Buffer pH 9.5 containing 0.1% STPP), determined at pH 9.5 with casein as substrate.
DETAILED DISCLOSURE OF THE INVENTION
The present invention relates to microorganisms of the novel alkalophilic species Bacillus sp. AC13, represented by the type culture Bacillus sp. AC13, NCIMB 40482.
The strain Bacillus sp. AC13, NCIMB 40482, has been deposited on 3 Mar. 1992 according to the Budapest Treaty on the International Recognition of the Deposits of Microorganisms for the Purpose of Patent Procedures, at National Collections of Industrial and Marine Bacteria Ltd., 23 St. Machar Drive, Aberdeen AB2 1RY, Scotland, UK, under Accession No. NCIMB 40482.
The microorganisms of this invention are aerobic, rod shaped, spore forming bacteria, and therefore belonging to the genus Bacillus.
Morphologically they can be described as rods having a diameter of 0.6-0.9 .mu.m and a length of 4-8 .mu.m. The spores are ellipsoid to round, approximately 1.2.times.0.8 .mu.m, terminal swe
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Bisg.ang.rd-Frantzen Henrik
Dambmann Claus
Olsen Arne Agerlin
Outtrup Helle
Schulein Martin
Lambiris, Esq. Elias J.
Lankford , Jr. Leon B.
Novo Nordisk A S
Zelson Esq. Steve T.
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