Alkaline reagent solution for eliminating ammonia in an assay

Details Alkaline reagent solution for eliminating ammonia in an assay Alkaline reagent solution for eliminating ammonia in an assay

1996-06-20

1999-02-16

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 25, 435189, 435190, 435822, 435966, C12Q 126, C12Q 132, C12N 902, C12N 904

Patent

active

058719482

ABSTRACT:
The invention relates to an ammonia elimination reagent for use in an enzymatic assay of a biological substance which produces ammonia as the reaction product. The reagent is an alkaline solution of pH 9 to 11 and contains Sulfolobus-derived thermostable isocitrate dehydrogenase, 2-oxoglutaric acid, a reducing coenzyme, isocitric acid, glutamate dehydrogenase, and a divalent metal salt. The reagent can be stored in solution and has excellent stability under conditions of alkaline pHs.

REFERENCES:
patent: 4742001 (1988-05-01), Marui et al.
patent: 5258286 (1993-11-01), Marui et al.
patent: 5336608 (1994-08-01), Niimura et al.
Danson, M.J. and Wood, P.A. Isocitrate dehydrogenase of the thermoacidophilic archaebacterium Sulpholobus acidocaldarius, FEBS Lett., 172:289-293, 1984.
Fujita, T., Takata, S. and Sunagara, Y. Enzymatic rate assay of creatinine in serum and urine, Clin. Chem. 39:2130-2136, 1993.
Abstract of Japan, JP-A-02 255 098, Oct. 15, 1990.
Abstract of Japan, JP-A-63 214 182 Sep. 6, 1988.
Fossati et al, "A step forward in enazymatic measurement of creatinine", Clinical Chemistry, vol. 40, No. 1 1994, pp. 130-137.
Chemical Abstracts (1984) 100:188433d cited in the examination of USSN 06/878561 which is the original USSN of US Patent No. 5,258,286.

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