Alkaline pullulanase Y having .alpha.-amylase activity

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435832, C12N 944, C12N 100

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active

051477963

ABSTRACT:
An isolated alkaline pullulanase Y having .alpha.-amylase activity; a microorganism producing the alkaline pullulanase Y; and a process for producing the alkaline pullulanase Y are disclosed. The alkaline pullulanase Y having .alpha.-amylase activity has its optimum pH at higher alkaline range than conventional alkaline pullulanases and exhibits excellent stability in a wide pH range. Further, the alkaline pullulanase Y has strong resistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. Thus the alkaline pullulanase Y can advantageously be used as a detergent component.

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Fems Microbiology Letters, vol. 20, 1983, pp. 55-59, Elsevier; C. T. Kelly et al.: "Extracellular alpha-glucosidase of an alkalophillic microorganism Bacillus sp. ATCC 21592" *p. 55, summary; pp. 57-58, paragraph: Results and discussion*.
Biochimica et Biophysica Acta, vol. 397, 1975, pp. 188-193, Elsevier Scientific Publishing Co., Amsterdam, NL; N. Nakamura et al.: "Purification and some properties of alkaline pullulanase from a strain of bacillus No. 202-1, an alkalophillic microorganism".

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