Alkaline protease from Bacillus proteolyticus species

Details Alkaline protease from Bacillus proteolyticus species Alkaline protease from Bacillus proteolyticus species

1995-02-17

1996-10-15

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435220, 510320, 510321, 510392, 510393, 510530, C12N 954, C12N 952, C11D 742

Patent

active

055653480

ABSTRACT:
An alkaline protease which is suitable for detergent formulations is produced by three strains of a species of Bacillus proteolyticus. The alkaline protease has the amino acid terminal sequence of Seq. ID NO.: 1 as follows: Ala-Gln-Ser-Val-Pro-Trp-Gly-Ile-Ser-Arg-Val-Gln-Ala-Pro-Ala-Ala-His-Asn-Ar g-Gly-. In addition, the alkaline protease has a molecular weight of 28 kdaltons, an isoelectric point from 10-11.5, an optimum pH for proteolytic activity at a pH in the range of 8.5 and 11.5, and retains at least 70% of its original activity after being held at a pH of 8.0 at a temperature of 43.degree. C. for a period of 11 days.

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