Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1993-02-22
1994-10-25
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435220, 25217412, 252DIG12, C12N 952, C12N 954, C11D 3386
Patent
active
053588657
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
This invention is in the field of detergent proteases derived from strains of alkalophilic Bacillus sp. More specifically, the invention is directed towards a novel alkaline protease derived from a strain of Bacillus sp. J 20. Moreover, the invention is directed towards a process for the preparation of the protease, the use of the protease as detergent enzyme, as well as detergent compositions and detergent additives comprising the protease of the invention.
BACKGROUND ART
Detergent enzymes have been marketed for more than 20 years and are now well established as normal detergent ingredients in both powder and liquid detergents all over the world. With the trend toward lower temperature washing, detergent enzyme consumption has increased during late years. Enzymes used in washing formulations comprise proteases, lipases, amylases, cellulases, as well as other enzymes, or mixtures hereof. Commercially most important are proteases.
Detergent proteases have been developed by isolation of proteases found in nature followed by testing in detergent formulations. Most detergent proteases are obtained from members of the genus Bacillus. Currently new types of proteases enter the market, offering the possibility of giving a better cost/performance ratio at various specified conditions.
Examples of commercial protease products are ALCALASE.TM., ESPERASE.TM. and SAVINASE.TM., all supplied by Novo Nordisk A/S, Denmark. These and similar enzyme products from other commercial sources are active in detergent solutions, i.e. at pH values in the range from 8 to 11 and in the presence of sequestering agents, surfactants and bleaching agents such as sodium borate. The ALCALASE.TM. protease is produced by strains of the species Bacillus licheniformis. The ESPERASE.TM. and SAVINASE.TM. proteases are obtained by cultivation of strains of alkalophilic Bacilli.
SUMMARY OF THE INVENTION
According to the present invention there are provided novel detergent proteases with excellent washability.
In its first aspect, the invention provides a protease having an apparent molecular weight of 29 kD; pI around 8.8; pH optimum in the range of from pH 10 to 12 (at 25.degree. C.); temperature optimum in the range of from 45.degree. to 65.degree. C. (at pH 9.5).
In another aspect, the invention provides a protease having an apparent molecular weight of 29 kD; pI around 8.8; pH optimum in the range of from pH 10 to 12 (at 25.degree. C.); temperature optimum in the range of from 45.degree. to 65.degree. C. (at pH 9.5); and immunochemical properties identical or partially identical to those of a protease derived from Bacillus sp. J 20, NCIMB No. 40262. In a more specific aspect, the protease is obtainable from a strain of Bacillus sp. J 20. In a yet more specific aspect, the protease is obtainable from Bacillus sp. J 20, NCIMB No. 40262, or a mutant or a variant thereof.
In a third aspect, the invention provides an isolated biologically pure culture of a strain of Bacillus sp. J 20. In a more specific aspect, a strain of Bacillus sp. J 20, NCIMB No. 40262, or a mutant or a variant thereof, is provided.
In a fourth aspect, the invention provides a process for the preparation of the protease, which process comprises cultivation of a protease producing strain of Bacillus sp. J 20 in a suitable nutrient medium, containing carbon and nitrogen sources and inorganic salts, followed by recovery of the desired enzyme. In a more specific aspect, Bacillus sp. J 20, NCIMB No. 40262, or a mutant or a variant thereof, is cultivated.
In a fifth aspect, the use of the enzyme as detergent enzyme is claimed. In a more specific aspect, the invention provides a detergent composition comprising the protease. In another specific aspect, the invention provides detergent additives comprising the protease.
BRIEF DESCRIPTION OF DRAWINGS
The present invention is further illustrated by reference to the accompanying drawings, in which:
FIG. 1 shows the relation (% relative activity) between temperature and proteolytic activity of an enzyme according
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Aaslyng Dorrit A.
Dambmann Claus
Outtrup Helle
Lowney Karen A.
Novo Nordisk A S
Robinson Douglas W.
Weber Jon P.
Zelson Steve T.
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