Alkaline protease

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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C435S221000, C435S007100

Reexamination Certificate

active

07429642

ABSTRACT:
An alkaline protease having an amino acid with one or more amino acid residues selected from among those located at (a) position 15, (b) position 16, (c) position 166, (d) position 167, (e) position 187, (f) position 346, and (g) position 405 of the amino acid sequence of SEQ No; 1, or at positions corresponding to these positions, are the following amino acid residues, respectively: (a): histidine, (b): threonine or glutamine, (c): glycine, (d): valine, (e): serine, (f): arginine, and (g); and a gene encoding the alkaline protease.

REFERENCES:
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patent: 7101698 (2006-09-01), Sato et al.
patent: 1 209 233 (2002-05-01), None
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patent: 2004-122 (2004-01-01), None
patent: 2004-57195 (2004-02-01), None
patent: 2004-187669 (2004-07-01), None
patent: WO 98/56927 (1998-12-01), None
Seffernick et al. (J. Bacteriology, vol. 183, pp. 2405-2410, 2001).
Wells, Biochemistry, vol. 29, pp. 8509-8517, 1990.
K. Saeki, et al., Biochemical and Biophysical Research Communications, vol. 279, No. 2, pp. 313-319, “Novel Oxidatively Stable Subtilisin-Like Serine Proteases From AlkaliphilicBacillus spp.: Enzymatic Properties, Sequences, and Evolutionary Relationships”, 2000.
C. Schmidt-Danner, et al., Trends in Biotechnology, vol. 17, No. 4, XP 004162829, pp. 135-136, “Directed Evolution of Industrial Enzymes”, Apr. 1999.

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