Alkaline phosphatase isoenzyme determination

Details Alkaline phosphatase isoenzyme determination Alkaline phosphatase isoenzyme determination

1976-07-13

1977-06-21

Prescott, Arthur C.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204180S, 204299R, 23230B, G01N 2726

Patent

active

040309959

ABSTRACT:
Alkaline phosphatase isoenzymes are separated electrophoretically in agarose gels, or cellulose acetate membranes, equilibrated in a solution containing a low ionic strength buffer and a non-ionic detergent, such as Triton X-100. The electrophoretically separated isoenzymes are developed colorimetrically using 5-bromo-4-chloro-3-indolyl phosphate with a transphosphorylating buffer. Zones of enzyme activity appear as sharply-defined brilliant blue bands. The method is capable of resolving liver, bone, placental, intestinal and bile isoenzymes.

REFERENCES:
patent: 3554894 (1971-01-01), Zemel
patent: 3558459 (1971-01-01), Granstrand et al.
patent: 3607695 (1971-09-01), Schneider
patent: 3635808 (1972-01-01), Elevitch
patent: 3766047 (1973-10-01), Elevitch
patent: 3808118 (1974-04-01), Golias
patent: 3855094 (1974-12-01), Teppo

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