Alkaline phosphatase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...

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435196, C12N 900, C12N 916

Patent

active

058210954

ABSTRACT:
A novel alkaline phosphatase and a method for the production of the alkaline phosphatase are described. A method for detecting a ligand in a sample and a method for analyzing the sequence of nucleic acid in a sample using the alkaline phosphatase are also described.

REFERENCES:
Pizauro et al. "Osseous plate alkaline phosphatase is anchored by GPI" Brazilian J. Med. Biol. Res., vol. 27, pp. 453-456, 1994.
Dobozy et al. "Some Properties of Alkaline Phosphatase in Bacillus Species" Acta Microbiol. Acad. Sci. Hung., vol. 16, pp. 181-187, 1969.
Boyer, P.D., ed. The Enzymes, 3rd Edition, "Mammalian Alkaline Phosphatases" (1971) vol. 4, 417-447.
F.M. Hulett-Cowling and Campbell, L.L., Purification and Properties of an Alkaline Phosphatase of Bacillus licheniformis (1971) Biochemistry, vol. 10, No. 8, 1364-1371.
M. Nomoto, Ohsawa, M., Wang, H.L., Chen, C.C. and Yeh, K.W., Purification and Characterization of Extracellular Alkaline Phosphatase From an Alkalophilic Bacterium (1988) Agric. Biol. Chem., 52(7), 1643-1647.
F.M. Hulett, Stuckmann, K., Spencer, D.B., Sanopoulou, T., Purification and Characterization of the Secreted Alkaline Phosphatase of Bacillus licheniformis MC14: Identification of a Possible Precursor (1986) Journal of General Microbiology 132, 2387-2395.

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