Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1995-05-25
1998-04-21
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
426 10, 426 20, 426 28, 426 61, C12N 904, A23B 500
Patent
active
057416885
DESCRIPTION:
BRIEF SUMMARY
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation of PCT/DK95/00178 filed May 3, 1995, which is incorporated herein by reference.
FIELD OF INVENTION
The present invention relates to a novel glucose oxidase, to a process for its production and to its use in bleaching and detergent compositions, as well as to its use as a dough strengthener.
BACKGROUND OF THE INVENTION
Glucose oxidases are enzymes that catalyze the oxidation of glucose with oxygen whereby hydrogen peroxide is formed. Such enzymes are known from microbial, plant and animal origins, e.g., glucose oxidase from Aspergillus, Penicillium and Talaromyces. Glucose oxidase has been described as useful for various purposes, e.g., for bleaching purposes and in the baking industry, useful for strengthening the dough.
An example of a commercial glucose oxidase is Gluzyme.TM., an Aspergillus niger glucose oxidase, available from Novo Nordisk A/S. This and similar products from other commercial sources have an acidic pH optimum, typically around pH 5, which means that they are not very active in detergent solutions due to the alkaline character of the detergents.
It is an object of the present invention to provide novel glucose oxidases with improved performance in neutral and alkaline solutions.
SUMMARY OF THE INVENTION
In this invention it is surprisingly found that a glucose oxidase with a neutral pH optimum may be produced from strains of Cladosporium.
Accordingly, the present invention relates to a glucose oxidase characterized by a pH-optimum in the range pH 6-7, having more than 75% of maximum activity at pH 8, determined at 30.degree. C. with D-glucose as substrate.
BRIEF DESCRIPTION OF DRAWINGS
The present invention is further illustrated by reference to the accompanying drawings, in which
FIG. 1 shows the relation between pH and the glucose oxidase activity of a novel glucose oxidase according to the invention (the glucose oxidase obtained according to Example 1), with D-glucose as substrate in the presence of oxygen at 30.degree. C., using a buffer system adjusted to predetermined pH values of from pH 4 to pH 9.
FIG. 2 shows the dynamic shear storage modulus G' in gluten from 4 different doughs characterized with the following symbols: oxidase from A. niger. of the invention, obtained as described in Example 1.
FIG. 3 shows the viscoelastic phase angle .delta. in gluten from 4 different doughs characterized with the following symbols: oxidase from A. niger. of the invention, obtained as described in Example 1.
DETAILED DESCRIPTION OF THE INVENTION
According to the invention, glucose oxidase is obtainable from a glucose oxidase producing strain of Cladosporium, preferably Cladosporium oxysporum.
The genus Cladosporium is characterized primarily by the formation of conidia in branching chains, which are very fragile and readily break up into units. The conidia can be either hyaline or pigmented, smooth or roughened, continuous or septate. The conidiophores are erect and pigmented, branching irregularly at the apex. Cladosporium oxysporum is further described by M. B. Ellis in Dermatiaceous Hyphomycetes, 1971, p. 312, CAB International, UK.
A strain representative of Cladosporium oxysporum has been deposited according to the Budapest Treaty on the International Recognition of the Deposits of Microorganisms for the Purpose of Patent Procedures, on 25 Mar. 1994, at Centraal-bureau voor Schimmelcultures (CBS), under Accession No. CBS 163.94.
Glucose oxidase of the invention may be produced by aerobic cultivation of the above mentioned microbial strain on a nutrient medium containing suitable carbon and nitrogen sources, such media being known in the art. A temperature in the range of from 20.degree. C. to 30.degree. C. is suitable for growth and glucose oxidase production.
Alternatively, glucose oxidase of the invention can be produced by aerobic cultivation of a transformed host organism containing the appropriate genetic information from the above mentioned strain. Such transformants can be prepared and culti
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Moore-Landecker, E. Fundamentals of the fungi p. 262, 1982.
Oji Paper Co., JP 6141854, Dialog Abs. No. 009937307 (1994).
Arica et al. J. Chem. Technol. Biotechnol. vol. 58(3) pp. 287-292. Abstract Enclosed.
Aagaard Jesper
Oxenb.o slashed.ll Karen M.
Qi Si Joan
Agris, Esq. Cheryl H.
Lankford , Jr. Leon B.
Novo Nordisk A S
Tafe Christopher R.
Zelson Esq. Steve T.
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