Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-06-01
1998-10-20
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435210, C12N 928, C12N 944
Patent
active
058245316
DESCRIPTION:
BRIEF SUMMARY
FIELD OF INVENTION
The present invention relates to amylases having improved dishwashing and/or washing performance.
BACKGROUND OF THE INVENTION
For a number of years .alpha.-amylase enzymes have been used for a variety of different purposes, the most important of which are starch liquefaction, textile desizing, starch modification in the paper and pulp industry, and for brewing and baking. A further use of .alpha.-amylases, which is becoming increasingly important is the removal of starchy stains during washing and dishwashing.
Examples of commercial .alpha.-amylase products are Termamyl.RTM., BAN.RTM. and Fungamyl.RTM., all available from Novo Nordisk A/S, Denmark. These and similar products from other commercial sources have an acidic to a neutral pH optimum, typically in the range of from pH 5 to pH 7.5, which means that they do not display optimal activity in detergent solutions owing to the alkaline character of the detergents.
It is an object of the present invention to provide novel .alpha.-amylase s with improved performance in alkaline solutions, especially in alkaline detergent solutions.
SUMMARY OF THE INVENTION
The present invention provides .alpha.-amylases with a very high specific activity at pH 8-10 and at temperatures of from 30.degree. C. to around 60.degree. C., conditions normal in detergent solutions.
Accordingly, the present invention relates to an .alpha.-amylase having a specific activity at least 25% higher than the specific activity of Termamyl.RTM. at a temperature in the range of 25.degree. C. to 55.degree. C. and at a pH value in the range of pH 8 to pH 10, measured by the .alpha.-amylase activity assay as described herein.
BRIEF DESCRIPTION OF DRAWINGS
The present invention is further illustrated with reference to the accompanying drawings, in which
FIG. 1 shows the relation between pH and the .alpha.-amylase activity of a novel amylase (obtained from Bacillus strain NCIB 12289), determined as described in Example 2.
FIG. 2 shows the pH profile of an .alpha.-amylase obtained from Bacillus strain NCIB 12512 (I), of an .alpha.-amylase obtained from Bacillus strain NCIB 12513 (II) and of Termamyl.RTM. (III) determined at 55.degree. C. in the pH interval of from 4 to 10.5, the test being performed as described in Example 3.
FIG. 3 shows the temperature profile of an .alpha.-amylase obtained from Bacillus strain NCIB 12512 (I), of an .alpha.-amylase obtained from Bacillus strain NCIB 12513 (II) and of Termamyl.RTM. (III) determined at pH 10.0 in the temperature interval of from 25.degree. C. to 95.degree. C., the test being performed as described in Example 3.
FIG. 4 shows the RSF-rating--removal of starch film from dish- and glassware, as a function of the dosage of a novel .alpha.-amylase (obtained from Bacillus strain NCIB 12289) at 55.degree. C., the test being performed as described in Example 4.
FIG. 5 shows the RSF-rating--removal of starch film from dish- and glassware, as a function of the dosage of a novel .alpha.-amylase (obtained from Bacillus strain NCIB 12512) at 45.degree. C. (.cndot.), at 55.degree. C. (*) and at 65.degree. C. (x), the test being performed as described in Example 4.
DETAILED DESCRIPTION OF THE INVENTION
One embodiment of the present invention provides an .alpha.-amylase having a specific activity at least 25% higher or at least 35% higher or at least 45% higher or at least 55% higher or at least 65% higher or at least 75% or at least 25-75% higher than the specific activity of Termamyl.RTM. at a temperature in the range of 25.degree. C. to 55.degree. C. or at a temperature in the range of 25.degree. C. to 35.degree. C. or at a temperature in the range of 35.degree. C. to 45.degree. C. or at a temperature in the range of 45.degree. C. to 55.degree. C. and at a pH value in the range of pH 8 to pH 10 or at a pH value in the range of pH 8 to 8.5 or at a pH value in the range of pH 8.5 to 9.0 or at a pH value in the range of pH 9.0 to 9.5 or at a pH value in the range of pH 9.5 to 10.0, measured by the .alpha.-amylase activity assay as desc
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Abstract--Dialog, File 155: Medline, 06517814 88162814.
Bisg.ang.rd-Frantzen Henrik
Outtrup Helle
Rasmussen Michael Dolberg
stergaard Peter Rahbek
Van der Zee Pia
Agris, Esq. Cheryl H.
Novid Nordisk
Stole Einar
Wax Robert A.
Zelson Esq. Steve T.
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