Alkaline agarosse gel electrophoresis of hemoglobins

Details Alkaline agarosse gel electrophoresis of hemoglobins Alkaline agarosse gel electrophoresis of hemoglobins

1979-06-01

1980-06-24

Prescott, Arthur C.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

23230B, 424 12, G01N 3316, G01N 2726

Patent

active

042093724

ABSTRACT:
The common hemoglobin variants A, A.sub.2, F, S, and C are discretely separated by alkaline agarose gel electrophoresis employing (1) an agarose gel having a wet thickness of from about 0.1 to about 0.5 mm., (2) a gel buffer comprising tris(hydroxymethyl)aminomethane, ethylenediaminetetraacetic acid, and boric acid, and having an ionic strength of from about 0.065 to about 0.1 and a pH of from about 8.7 to about 9.1, (3) a well buffer comprising diethylbarbiturate and ethylenediaminetetraacetate and having an ionic strength of from about 0.01 to about 0.11 and a pH of from about 8.0 to about 9.5, and (4) a potential of from about 150 to about 300 volts.

REFERENCES:
patent: Re24752 (1959-12-01), Ressler
patent: 3497437 (1970-02-01), Louderback et al.
patent: 3558459 (1971-01-01), Granstrand et al.
patent: 3607695 (1971-09-01), Schneider
patent: 3692654 (1972-09-01), Svendsen

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