Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1991-06-11
1993-03-09
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435219, C12N 952, C12N 950
Patent
active
051926776
ABSTRACT:
An exocellular protease from Thermomonospora fusca YX and a process for producing the protease which has the following physicochemical properties:
(1) Molecular mass:
The protease has a molecular mass of from about 10,000 to 14,000 Daltons as measured by SDS-polyacrylamide gel electrophoresis;
(2) Influence of inhibitors:
The protease activity is inhibited by serine protease inhibitors;
(3) Substrate specificity:
A non-specific protease which can hydrolyze food proteins and bovine serum albumin at a rate of 50-100 nmoles peptide bonds/.mu.g enzyme/minute at 55.degree., pH 8.5 in 0.5 M Tris buffer without showing any substrate and/or product inhibition;
(4) Reactivity:
A broad spectrum serine type protease having activity at least 5 times greater than trypsin or chymotrypsin towards food grade proteins and bovine serum albumin;
(5) Optimum activity temperature and temperature range:
The optimum activity temperature is 80.degree. C. at pH of 8.0 in 0.05 M Tris buffer at an ionic strength of 0.2 M NaCl; The temperature range is 35.degree. to 95.degree. C. under the same pH, buffer, and ionic strength;
(6) pH range and optimum pH value:
The protease has a pH activity range of from about 7 to 11, and the optimum pH value is 9.0;
(7) Tolerance to ionic strength conditions and optimum ionic strength:
The protease is tolerant to ionic strengths of from 0.0 to about 1.0 M NaCl; Optimum ionic strength is 0.2 M NaCl;
(8) Isoelectric point:
The isoelectric point is at an alkaline pH; and
(9) Structure:
The protease is a monomer.
REFERENCES:
patent: 3692631 (1972-09-01), Larson et al.
patent: 4364926 (1982-12-01), Yokogawa et al.
patent: 4480036 (1984-10-01), Morgan et al.
Upton, M. E. et al. (1977) Appl. Environ. Michrobiol. 33(1), 59-64.
Stutzenberger, F. J., et al. (1982) Chem. Abst. 96:212963y.
Stutzenberger, F., Journal of Bacteriology, Jun. 1987, pp. 2774-2780.
Stutzenberger & Lawson, Biotechnology and Bioengineering, vol. XXIV, pp. 999-1006, (1982).
McCarthy & Cross, Journal of General Microbiology (1984), 130, 5-25.
Kristjansson, M. M., et al. (1990) Int. J. Peptide Protein Res. 29, preprint.
Gusek Todd W.
Kinsella John E.
Wilson David B.
Cornell Research Foundation Inc.
Patterson Jr. Charles L.
LandOfFree
Alkali and heat stable protease from thermomonospora fusca does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Alkali and heat stable protease from thermomonospora fusca, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Alkali and heat stable protease from thermomonospora fusca will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-210790