Alga-origin promoter, intron and terminator

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C435S320100, C800S278000

Reexamination Certificate

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07449568

ABSTRACT:
A promoter, an intron and a terminator by which a useful foreign gene can be efficiently expressed in algae and which are thus useful in breeding algae using genetic engineering techniques.

REFERENCES:
Hawkins et al. 1999 Current Microbiology 38:335-341.
Kim et al. 1994, Plant Molecular Biology 24: 105-117.
Fourgoux-Nicol et al. 1999, Plant Molecular Biology 40 :857-872.
Fukuda, S. et al “Isolation, characterization and expression of a cDNA encoding an elongation factor-1α fromPorphyra yezoensis(Bangiales, Rhodophyta)” Phycological Research vol. 50 2002 pp. 11-15.
Kuwano, K. et al “Cryopreservation of clonal gametophytic thalli ofPorphyra(Rhodophyta)” Plant Science vol. 116 1996 pp. 117-124.
Lee, E.K. et al “Analysis of Expressed Sequence Tags ofPorphyra yezoensis” Molecules and Cells, vol. 10 No. 3, pp. 338-342.
Liu, Q.Y. et al “Elongation factor 1α genes of the red algaPorphyra purpureaInclude a novel, developmentally specialized variant” Plant Molecular Biology, vol. 31, 1996 pp. 77-85.
Nikaido, I. et al “Generation of 10,154 Expressed Sequence Tags from a Leafy Gametophyte of a Marine Red Alga,Porphyra yezoensis” DNA Researchvol. 7 2000 pp. 223-227.
Tanaka, A. et al “Enhancement of foreign gene expression by a dicot intron in rice but not in tobacco is correlated with an increaded level of mRNA and an efficient splicing of the intron” Nucleic Acids Research, vol. 18, No. 23 pp. 6767-6770.

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