Aldicarb immunoassay by sulfone equivalents

Details Aldicarb immunoassay by sulfone equivalents Aldicarb immunoassay by sulfone equivalents

1991-07-16

1993-04-06

Ceperley, Mary E.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

435 793, 436531, 436538, 436815, G01N 33545

Patent

active

052003469

ABSTRACT:
An immunoassay for detecting total aldicarb in an aqueous sample suspected of containing the same is disclosed. In the inventive method, an oxidant is added to the aqueous sample, with the oxidant provided in an amount sufficient to oxidize all aldicarb contained in the solution to aldicarb sulfone. Next, the aqueous sample is combined with a thioether solution capable of neutralizing excess oxidant in the aqueous sample to thereby provide a neutralized test solution. The neutralized test solution is then contacted to an antibody capable of binding aldicarb sulfone, and the aldicarb sulfone bound to the antibody detected.

REFERENCES:
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H. Dorough et al., J. Chromatographic Science, vol. 13, pp. 212-224 (1975).
B. Trost et al., J. Org. Chem., 53, 532-537 (1988), Tetra-n-butylammonium Oxone. Oxidations under anhydrous conditions.
J. Hawk et al., Analytical Chemistry, 44, 1315-1317 (1972), Kinetic method for quantitative determination of individual organic peroxides in peroxide mixtures.
J. Brady et al., ACS Symposium Ser., 382 (Biol. Monit. Pestic. Exposure: Meas., Estim., Risk Reduction), 262-284 (1989), Chap. 21, Enzyme immunoassay for aldicarb.

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