Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1994-10-14
1997-05-27
Fox, David T.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 691, C12N 1500, C12N 504, C12N 1582, A01H 500
Patent
active
056331533
ABSTRACT:
The instant application provides a method of plant transformation in which plant cells are transformed with an aldehyde dehydrogenase gene capable of detoxifying a phytotoxic aldehyde selective agent. Transformed plant cells are cultured in the presence of the phytotoxic aldehyde selective agent to produce transformed shoots which may be regenerated to produce transformed plant cells containing the aldehyde dehydrogenase gene. The aldehyde dehydrogenase gene construct is linked to another gene construct of interest for expression in plant cells, wherein the aldehyde dehydrogenase gene acts as a selectable marker for transgenic plant cells containing the desired gene construct.
REFERENCES:
Li et al (1992) Plant Physiol 100:662-668.
Boyd, et al., "Characterization of an Escherichia Coli Gene Encoding Betaine Aldehyde Dehydrogenase (BADH) Structural Similarity to Mammalian ALDHs and a Plant BADH", Gene (1991), 103 45-52.
Holmstrom, et al., "Production of the Escherichia coli Betaine-Aldehyde Dehydrogenase, an Enzyme Required for the Synthesis of the Osmoprotectant Glycine Betaine, in Transgenic Plants" The Plant Journal (1994) 6(5) 749-758.
McCue, et al "Salt-Inducible Betaine, Aldehyde Dehydrogenase from Sugar Beet: cDNA Cloning and Expression" Plant Molecular Biology (1992) 18 1-11.
Rathinasabapathi, et al., "Matabolic Engineering of Glycine Betaine Synthesis: Plant Betaine Aldehyde Dehydrogenases Lacking Typical Transit Peptides are Targeted to Tobacco Chloroplasts where they Confer Betaine Aldehyde Resistance" Planta (1994) 193 155-162.
Weretilnyk, et al., "Molecular Cloning of a Plant Betaine-Aldehyde Dehydrogenase, an Enzyme Implicated in Adaptation to Salinity and Drought" Proc. Nat. Acad. Sci. (1990) vol. 87 2745-2749.
Calgene Inc.
Fox David T.
McElwain Elizabeth F.
Spruill W. Murray
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