Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1997-02-06
1998-07-07
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435136, 435190, 4352521, 435822, C12P 760
Patent
active
057767424
ABSTRACT:
An aldehyde dehydrogenase having the physico-chemical properties: molecular weight: 91,000.+-.5,000; substrate specificity:active on aldehyde compounds; inhibition: by Cu.sup.2+, Zn.sup.2+, Ni.sup.2 + and ethylenediamine tetraacetic acid; optimum pH: 6.0-8.5; optimum temperature: 20.degree.-40.degree. C.; and stimulator: Ca.sup.2+ and pyrroloquinoline quinone, is derived from a microorganism belonging to the genus Gluconobacter. Said aldehyde dehydrogenase can be produced by cultivating a microorganism of the genus Gluconobacter which is capable of producing an aldehyde dehydrogenase having the above properties, in an aqueous nutrient medium under aerobic conditions, disrupting the cells of the microorganism and isolating and purifying the aldehyde dehydrogenase from the cell-free extract of the disrupted cells of the microorganism. The aldehyde dehydrogenase is useful for the preparation of 2-keto-L-gulonic acid (2-KGA) which can be produced from L-sorbosone by contacting L-sorbosone with (i) the aldehyde dehydrogenase in the presence of an electron acceptor, (ii) a Gluconobacter microorganism capable of producing the aldehyde dehydrogenase in an aqueous medium under aerobic conditions or (iii) a cell-free extract of said microorganism, and in each case isolating the resulting 2-keto-L-gulonic acid from the reaction mixture.
REFERENCES:
patent: 3907639 (1975-09-01), Makover et al.
patent: 5437989 (1995-08-01), Asakura et al.
Hoshino, T., et al., Agric. Biol. Chem., 55(3):665-670 (1991).
Kitamura, I., et al., Eur. J. Appl. Microbiol., 2:1-7 (1975).
Makover, S., et al., Biotechnol. Bioeng., 17:1485-1514 (1975).
Hoshino Tatsuo
Sugisawa Teruhide
Johnston George W.
Kirk, Jr. Joseph P.
Lilling Herbert J.
Roche Vitamins Inc.
Tramaloni Dennis P.
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