Alcohol oxidase from Pichia-type yeasts

Details Alcohol oxidase from Pichia-type yeasts Alcohol oxidase from Pichia-type yeasts

1985-07-09

1986-10-28

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C12N 904

Patent

active

046198989

ABSTRACT:
A new alcohol oxidase is isolated from Pichia-type microorganisms in soluble or crystalline form. The crystalline novel enzyme is isolated by preparing an aqueous fluid containing cells of a Pichia-type microorganism, homogenizing the fluid and separating solids therefrom to produce an alcohol oxidase solution, adjusting the solution to have an ionic strength in the range of 0.05 to 0.01 in ionic strength to form a recovery range solution thereby causing the crystalline alcohol oxidase to form. The new enzyme is used to determine alcohol concentrations in fluid samples in which conditions are compatible with the enzyme's activity.

REFERENCES:
Kato et al., "Enzyme System for Methanol Oxidation in Yeasts," Agr. Biol. Chem., 38(3), 1974, pp. 675-677.
Fujii et al., "Oxidation of Methanol, Formaldehyde and Formate by a Candida Species," Agr. Biol. Chem., 36(13), 1972, pp. 2297-2306.
Tani et al., "The Microbial Metabolism of Methanol," Agr. Biol. Chem., 36(1), 1972, pp. 76-83.
Kato et al., "Alcohol Oxidases of Kloeckera sp. and Hansenula polymorpha," Eur. J. Biochem., 64, 1976, pp. 341-350.
Tani et al., "Utilization of Methanol by Yeasts," Advances in Applied Microbiology, 24, 1978, pp. 165-186.
Yamada et al., "Purification and Characterization of Alcohol Oxidase from Candida 25-A," Agric. Biol. Chem., 43(4), 1979, pp. 877-878.
Lee et al., "Taxonomic Study of Methanol Assimilating Yeasts", J. Gen. Appl. Microbiology, vol. 26, pp. 133-158 (1980).

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