Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1993-12-03
1995-08-01
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
435136, 4352521, 435822, C12N 904, C12N 112, C12P 740
Patent
active
054379890
ABSTRACT:
A homogeneous alcohol/aldehyde dehydrogenase isolated from Gluconobacter oxydans DSM 4025 FERM BP-3812 is disclosed. The enzyme is capable in the presence of an electron acceptor, of catalyzing the conversion of L-sorbose to 2-keto-L-gulonic acid via L-sorbosone. The enzyme has a molecular weight of 130,000-140,000 daltons as determined by gel filtration column chromatography, a pyrroloquinoline quinone prosthetic group, an optimum pH for enzyme activity of 7.0 to 9.0, an optimum temperature for enzyme activity from about 20.degree. C. to 40.degree. C. and an isoelectric point of about 4.4. Also disclosed is a process for producing the enzyme and a process for producing aldehydes, carboxylic acids and ketones, especially 2-keto-L-gulonic acid utilizing the enzyme.
REFERENCES:
patent: 4352885 (1982-10-01), Zeikus
Ghosh, et al, J. Biochem. 199, 245-250 (1981).
Groen, et al, J. Biochem. 223, 921-924 (1984).
Goren, et al, J. Biochem. 234, 611-615 (1986).
Anthony, Advances in Microbial Phys, 27 113-209 (1986).
Asakura Akira
Hoshino Tatsuo
Epstein William H.
Gould George M.
Hoffmann-La Roche Inc.
Meller Michael V.
Naff David M.
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