Albumin-dye complex for fatty acid determination

Details Albumin-dye complex for fatty acid determination Albumin-dye complex for fatty acid determination

1977-10-04

1980-03-25

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

23230B, 23902, 23909, 260121, 424 2, 435 18, 435 19, G01N 3114, G01N 3316, C07G 700

Patent

active

041951263

ABSTRACT:
An albumin-dye complex is prepared containing serum or plasma albumin which is substantially free of endogenous fatty acids and which has been complexed with a fatty acid and dye which dye may be displaced from the albumin-dye complex by free fatty acids. The complex is used to quantitatively determine fatty acids, compounds which hydrolyze to fatty acids or activity of hydrolase enzymes that produce fatty acids from a substrate.

REFERENCES:
patent: 3703591 (1972-11-01), Bucolo et al.
patent: 3751381 (1973-08-01), Megram
patent: 3983005 (1976-09-01), Goodhue et al.
patent: 4038146 (1977-07-01), Nonatz et al.
patent: 4043997 (1977-08-01), Schroeder
patent: 4066508 (1978-01-01), Ranscher et al.
patent: 4093612 (1978-06-01), Travis et al.
Irving et al., The Binding of Organic Ions by Proteins, J. Amer. Chem. Soc., vol. 68, 1946, (pp. 1486-1490).
Irving, M. K., Spectrophotometric Investigations of the Interactions of Proteins with Organic Anions, J. Amer. Chem. Soc., vol. 68, 1946, (pp. 2299-2304).
Daniel et al., Cooperative Effects in Binding by Bovine Serum Albumin, Biochemistry, vol. 5, No. 6, 1966, (pp. 1893-1900).

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