Air cleaning filter

Gas separation: apparatus – With sterilizing means – Means uses chemical antimicrobial agent

Reexamination Certificate

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Details

C422S004000, C422S028000, C442S123000, C435S176000, C435S177000, C435S180000

Reexamination Certificate

active

06579352

ABSTRACT:

TECHNICAL FIELD
This invention relates to an air purifying filter having an enzyme immobilized on a surface of a carrier. The invention particularly relates to an air purifying filter having an enzyme immobilized on a surface of a HEPA filter which is composed of boron-silica glass fibers not rendered to be water repellent, ion-exchange fibers having functional groups or boron-silica glass fibers coated with a polymer having functional groups.
BACKGROUND ART
Various air purifiers (air cleaners) and air washers are known as apparatus for removing unwanted materials from air. Air purifiers (air cleaners) are apparatuses that primarily depend on air purifying filters (air filters) for filtering off unwanted materials in air such as suspended fine particles (e.g. dust) on which gaseous contaminants, fats/oils and microorganisms such as bacteria are occasionally deposited. Air washers are apparatus which typically involve the washing of air with water to remove dirt/dust particles, microorganisms, etc. from air.
The first mentioned air purifying filters are available in various types which have been developed to suit specific factors such as the material to be removed, its particle size and the efficiency of particle capture. The filters are also available in various shapes including mats, wedges, fold-ins, baskets, bags, panels and boxes. However, single use of the conventional air purifying filters has been unable to achieve complete removal of air-borne microorganisms such as molds, bacteria and fungi. Further, the microorganisms captured on the filters are difficult to control or kill and may grow on the filters and scatter about to cause secondary contamination; therefore, the existing air purifying filters have not necessarily given satisfactory results in the air purification treatment.
Under the circumstances, the present inventors thought of using enzymes effective in controlling or killing air-borne microorganisms such as mold, bacteria and fungi. Various techniques have so far been proposed as bacteriacidal/sterilizing or antimicrobial means using enzyme and they include the following.
Japanese Patent Publication No. 21422/1979 discloses a method of controlling microorganisms (e.g. heat-resistant cell spores) in processed food which is characterized in that at least one member of the group consisting of urea, thioglycolic acid and mercaptoethanol, and a bacterial cell wall lysing enzyme are added to the food or starting materials thereof and the mixture is held for a specified time and subsequently heat treated.
Japanese Patent Public Disclosure No. 30584/1989 describes a biocatalyst immobilizing carrier for typical use in the food industry which has a microorganism or an enzyme that work as a biocatalyst immobilized on a carrier together with a lysing enzyme. According to the patent, exemplary microorganisms include Gram-negative bacteria, Gram-positive bacteria, yeasts and molds; useful enzymes are hydrolases such as amylase, protease and lipase; lysing enzymes include lysozyme, endo-N-acetylmuramidase, endo-N-acetylglucosaminidase, autolysin, ensopeptidase-type bacterial cell wall lysing enzymes, amidase-type bacterial cell wall lysing enzymes, mold cell wall lysing enzymes and yeast cell wall lysing enzymes.
Japanese Patent Public Disclosure No. 5822/1990 discloses an alcoholic preparation for use on raw vegetable which incorporates egg white lysozyme as a natural antimicrobial agent and which has the pH adjusted to be within the range of 2.0-7.0, as well as a modifier for use on raw vegetable which incorporates an organic acid and an organic acid salt or a phosphate salt, has the pH adjusted to be within the range of 2.0-7.0 and also incorporates egg white lysozyme as an antimicrobial agent.
Japanese Patent Public Disclosure No. 23856/1990 discloses a food preservation method which, for the purpose of effectively preventing putrefaction and deterioration of food, adds a polyglycerin fatty acid ester, lysozyme and protamine to the food being produced.
Japanese Patent Publication No. 22144/1991 describes a method of food sterilization which comprises allowing a lysing enzyme such as lysozyme, kinase or &bgr;-1,6-glucanase to act upon food and then subjecting it to an ultrasonic treatment.
Japanese Patent Public Disclosure No. 76362/1993 describes a process for producing lysozyme-containing particles which comprises the steps of applying a spray of an aqueous lysozyme slurry into the reaction chamber of a fluidized-bed reactor together with the core particles of a hydratable substance, evaporating the residual water so that the dry lysozyme coating will remain on the particulate core substance, to thereby provide lysozyme-containing particles. The patent teaches that the thus produced lysozyme-containing particles are useful in various kinds of foods, cosmetics, medicines and other applications.
Japanese Patent Public Disclosure No. 276910/1993 discloses a food preservative characterized by the combination of protamine with at least one substance selected from the group consisting of lysozyme, an antimicrobial substance extracted from licorice, vitamin B
1
ester and a polyphopshate salt.
Japanese Patent Public Disclosure No. 217749/1994 discloses a food preservative which comprises a caprylic acid monoglyceride and/or a capric monoglyceride in combination with glycine, sodium acetate, lysozyme and an organic acid or an alkali salt thereof, as well as a food preservative which comprises a caprylic acid monoglyceride and/or a capric monoglyceride in combination with glycine, sodium acetate, lysozyme and polyphosphate salt. The patent also teaches that although lysozyme is said to have a lysing effect, its effectiveness is limited to some microbial strains and it cannot be claimed as a practically feasible bacteriostat if used alone.
Japanese Patent Public Disclosure No. 246157/1994 discloses a cell adsorber which has the denatured product of a protein such as lysozyme, avidin or trypsin immobilized on a water-insoluble carrier. According to the patent, cells can be effectively separated or removed from a cell-containing solution by using the cell adsorber.
Japanese Patent Public Disclosure No. 236479/1995 discloses the lysozyme binding of an antimicrobial compound selected from among plant-derived antimicrobial compounds (e.g. perillaldehyde, cinnamaldehyde, salicylaldehyde, anisaldehyde, benzaldehyde and vanillin), antibiotics, synthetic antimicrobial agents, etc. The patent teaches that lysozyme bound to these antimicrobial compounds is useful in medicines, quasi-drugs, foods, etc.
There have also been proposed various inorganic antimicrobial materials which have antimicrobial metals such as silver, zinc and copper supported on inorganic carriers. Examples are inorganic antimicrobial agents having silver, zinc and other ions supported on zeolite through ion-exchange, inorganic antimicrobial agents having metallic silver supported on calcium phosphate through adsorption, inorganic antimicrobial agents having silver ions supported on zirconium phosphate through ion-exchange, and inorganic antimicrobial agents having silver complex salts supported on amorphous silicon oxide through occlusion; these inorganic antimicrobial materials are applied to fibers, plastics, films, paints and various other products (ZEOLITE, vol. 13 No. 2 (1966), pp. 56-63).
Various carriers have also been proposed for use in immobilizing the aforementioned enzymes and they include the following.
Japanese Patent Public Disclosure No. 48825/1974 describes the use of phenolic and aliphatic amine-based ion-exchange resins as carriers for immobilizing egg white lysozyme.
Japanese Patent Public Disclosure No. 48080/1984 discloses the use of platinum coated with Amberlite or aminated polyvinyl alcohol and the like for complex immobilizing of more than one enzyme.
Japanese Patent Public Disclosure No. 49795/1985 discloses the use of nonwoven carriers composed of natural fibers or chemical fibers or mixtures thereof in the web for immobilization treatment of bactericidal lysing enzymes.
Japanese Patent Pu

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