AHAS promoter useful for expression of introduced genes in plant

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

800DIG56, 536 236, 536 241, 4351723, 4352404, 4353201, C12N 1505, C12N 1529, C12N 1582, A01H 500

Patent

active

057508666

ABSTRACT:
The present invention is directed to isolated non-coding nucleotide sequences useful as promoters for heterologous gene expression in plants. The present invention is also directed to vectors and plant cells comprising the isolated nucleotide sequences.
AHAS promoters from maize are used to express introduced genes at high levels and in various plant tissues. Promoters from the als1 and als2 genes of maize are cloned and sequenced, and the promoter regions from these genes are then introduced into a plasmid 5' to the reporter gene beta-glucuronidase (GUS). Both promoter fragments are from the XI12 maize line. The als1 promoter fragment is approximately 1400 base pairs long, whereas the als2 promoter fragment contains 829 base pairs.

REFERENCES:
patent: 4943674 (1990-07-01), Houck et al.
Fang et al. Sequence of Two Acetohydroxyacid Synthase Genes from Zea Mays. Plant Molecular Biology 18: 1185-1187, 1992.
McElroy et al. The Plant Cell vol. 2, 163-171 Feb. 1990.
Gordon-Kamm et al. The Plant Cell vol. 2, 603-618, Jul. 1990.
McElroy, David, Construction of Expression Vectors based on the rice actin1(Act1) 5'Region for use in Monocot Transformation, Mol. Gen Genet (1991) 231:150-160.
Zhang, W., Analysis of Rice Act 1 5'Region Activity in Transgenic Rice Plants, The Plant Cell, vol. 3, 1155-1165, Nov. 1991.
Christensen, A.H., Maize Polyubiquitin genes: structure, thermal pertubation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation, Plant Molecular Biology 18:675-689,(1992).
Kyozuka, J., Anaerobic induction and tissue-specific expression of maize Adh1 promoter in transgenicrice plants and their progeny, Mol, Gen Genet (1991)228:40-48.
Planckaert, F., Transient gene expression after electroporation of protoplasts derived from embryonic maize callus, Plant Cell Reports (1989) : 144-147.
McElroy, D., Foreign Gene Expression in Transgenic Cereals, Tibtech, Feb. 1994 (vol. 12).
Christensen, A.H., Sequence Analysis and Transcriptional Regulation by Heat Shock of Polyubiquitin Transcripts from Maize, Plant Molecular Biology 12: 619-632 (1989).
Reynolds, G. J., cDNA cloning of a tetraubiquitin gene, and expression of ubiquitin-containing trascripts, in aleurone layers of Avena fatua, Plant Molecular Biology, 20: 753-758 (1992).
Callis, J., Introns Increase Gene Expression in Cultured Maize Cells, Genes & Development 1:1183-1200 (1987).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

AHAS promoter useful for expression of introduced genes in plant does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with AHAS promoter useful for expression of introduced genes in plant, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and AHAS promoter useful for expression of introduced genes in plant will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-981795

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.