Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1992-05-26
1994-10-11
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
4353201, C12N 121, C12N 1574
Patent
active
053546849
ABSTRACT:
The present invention provides transfer-deficient pAgK84 plasmids, non-pathogenic strains of Agrobacterium radiobacter K84 carrying such plasmids, and a method useful for control of crown gall disease mediated by A. tumefaciens. The plasmids include genes encoding the synthesis of the antibiotic agrocin 84 and are modified in the transfer region of the plasmid by sufficient deletion to inhibit transfer of the plasmid. The plasmids are used to develop non-pathogenic strains of A. radiobacter K84 that stably produce agrocin 84 and inhibit the development of crown gall disease.
REFERENCES:
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Shim et al. Phytopathology. 1987. vol. 77(3):463-6. Abstract.
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Daniel McAlpine Memorial Lecture; Agrobacterium: pathogen, genetic engineer and biological control agent; The Australasian Plant Pathology, vol. 16(3) 1987.
Construction of a Tra deletion mutual of pAgK84 to safeguard the biological control of crown gall; The Jones et al reference (Mol. Gen. Genet.) (1988)
Biological Abstracts vol. 80, No. 2, Abstract No. 12493.
Genetic identification of functions of TL-DNA transcripts in octopine crown galls; The Leemans et al reference (The EMBO Journal vol. 1, pp. 147-152, 1982).
Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of the Ti plasmids of Agrobacterium Tumefaciens; The Haute et al reference (The EMBO Journal vol. 2, No. 3, pp. 411-417, 1983).
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Clare Bruce G.
Farrand Stephen K.
Jones David A.
Kerr Allen
Ryder Maarten H.
Choi Kathleen L.
Luminis Pty. Ltd.
Schwartz Richard A.
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