Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Via agrobacterium
Reexamination Certificate
2006-06-06
2006-06-06
Fox, David T. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Via agrobacterium
C800S279000, C800S281000, C800S284000, C800S288000, C800S320000, C435S430100, C435S469000
Reexamination Certificate
active
07057090
ABSTRACT:
A method of obtaining transgenic turfgrass plants by anAgrobacterium-mediated transformation protocol is disclosed. The protocol makes use of a modifiedAgrobacteriumvector system in which selectable marker genes and other genes of interest are operably linked to strong promoters from monocotyledenous plants, such as actin and ubiquitin promoters, that function efficiently in turfgrass cells. Transgenic turfgrass plants of several species, produced by theAgrobacterium-mediated transformation method, are also disclosed.
REFERENCES:
patent: 4810648 (1989-03-01), Stalker
patent: 5591616 (1997-01-01), Hiei et al.
patent: 5731179 (1998-03-01), Komari et al.
patent: 5948956 (1999-09-01), Lee et al.
patent: WO 98/06860 (1998-02-01), None
(Burch, Ann. Rev. Plant Physiology and Plant Mol Biol, vol. 48, pp. 297-326, 1997.
Deroles, SC and Gardner, RC; (1998) Plant Molecular Biology 11:355-364.
Dunwell, JM and Paul, EM, 1990, Outlook on Agriculture 19, 103-109.
Finnegan J and McElory D Bio/Technology 12:883-888, 1994).
Potrykus, I. Bio/Technology 8(6):535-542 (Jun. 1990).
Cheng et al. Plant Physiology 115:971-980 (1997).
Chawla, H.S. pp. 73-80 and 185-200 In: Plant Biotechnology, A Practical Approach, Science Publishers, Enfield NH (2003).
Komari, T. Plant Cell Reports 9: 303-306 (1990).
Aldemita and Hodges,Agrobacterium tumefaciens-Mediated Transformation ofjaponiandindicaRice Varieties ,Planta, Springer-Verlag 1996pp., 612-617.
Belanger et al., Turfgrass Biotechnology,Rutgers Turfgrass Proceedings,28: 1-3 (1996).
Cheng et al., Genetic Transformation of Wheat Mediated byAgrobacterium tumefaciens, Plant Physiol.(1997) 15:971-980.
Czernilofsky et al., Fate of Selectable Marker DNA Integrated into the Genome ofNicotiana Tabacum, DNA, vol. 5, No. 2, 1986, pp. 101-113.
de la Fuente et al., Aluminum Tolerance in Transgenic Plants by Alteration of Citrate Synthesis,Science, vol. 276, Jun. 6, 1997, pp. 1566-1568.
Hiei et al., Efficient Transformation of Rice (Oryza sativa L.) Mediated byAgrobacteriumand Sequence Analysis of the Boundaries of the T-DNA,The Plant Journal(1994), 6(2), 271-282.
Ishida et al., High Efficiency Transformation of Maize (Zea mays L.) Mediated byAgrobacterium tumefaciens, Nature Biotech.,vol. 14, Jun. 14, 1996, pp. 745-750.
Keller et al., A Plant Homolog of the Neutrophil NADPH Oxidase gp91phoxSubunit Gene Encodes a Plasma Membrane Protein with Ca2+Binding Motifs,The Plant Cell, vol. 10, 255-266, Feb. 1998.
Komari T., Transformation of Cluttered Cells ofChenopodium quinoaby Binding Vectors that Carry a Fragment of DNA from the Virulence Region of pTIBo542,Plant Cell Reports,(199) 9:303-306.
Komari et al., Vectors Carrying Two Separate T-DNAs for Co-Transformation of Higher Plants Mediated byAgrobacterium tumefaciensand Segregation of Transformants Free from Selection Markers,The Plant Journal,(1996) 10(1) 165-174.
Lee L., Turfgrass Biotechnology,Plant Science,15 (1996) 1-8.
Lodge et al., Broad-Spectrum Virus Resistance in Transgenic Plants Expressing Pokeweed Antiviral Protein,Proc. Natl. Acad. Sci. USA, vol. 90, pp. 7089-7093, Aug. 1993.
May et al., Generation of Transgenic Banana (Musa acuminata) Plants viaAgrobacterium-Mediated Transformation,Biotechnology,vol. 13, May 1995, pp. 486-492.
Meesters et al., Cloning and Expression of the Δ9Fatty Acid Desaturase Gene fromCryptococcuscurvatus ATCC 20509 Containing Histidine Boxes and a Cytochrome b5Domain,Appl. Microbiol. Biotechnol.,(1997) 47:663-667.
Mittler et al., Coordinated Activation of Programmed Cell Death and Defense Mechanisms in Transgenic Tobacco Plants Expressing a Bacterial Proton Pump,The Plant Cell,vol. 7, 29-42, Jan. 1995.
Stukey et al., TheOLE1Gene ofSaccharomyces cerevisiaeEncodes the Δ9Fatty Acid Desaturase and can be Functionally Replaced by the Rat Stearoyl-CoA Desaturase Gene,The Journal of Biol. Chem.,vol. 265, No. 33, Nov. 25, 1990, pp. 20144-20149.
Tingay et al.,Agrobacterium tumefaciens-Mediated Barley Transformation,The Plant Journal,(1997) 11(6), 1369-1376.
Wang et al., Expression of the Yeast Δ9Desaturase Gene in tomato Enhances its Resistance to Powdery Mildew,Physiological and Molecular Plant Pathology,(1998), 52, 371-383.
Wu et al., Disease Resistance Conferred by Expression of a Gene Encoding H2O2-Generating Glucose Oxidase in Transgenic Potato Plants,The Plant Cell,vol. 7, 1357-1368, Sep. 1995.
Zoubenko et al., Plant Resistance to Fungal Infection Induced by Nontoxic Pokeweed Antiviral Protein Mutant,Nature Biotechnology,vol. 15, Oct. 1997, pp. 992-996.
Chan, M.-T., et al., “Agrobacterium-mediated production of transgenic rice plants expressing a chimeric α-amylase promoter/ β-glucuronidase gene,”Plant molecular Biology, 1993, 22, 491-506.
Christensen, A.H., et al., “Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants,”Transgenic Res., 1996, 5, 213-218.
Dalton, S.J., et al.,Agrobacteriummediated transformation ofFestuca arundinaceaandLolium multiflorum, J. of Experimental Botany, 1998, 49(Suppl.), P4.26, p. 32.
Hiei, Y., et al., “Transformation of rice mediated byAgrobacterium tumefaciens,” Plant Molecular Biology, 1997, 35, 205-218.
Inokuma, C., et al., “Transgenic Japanese lawngrass (Zoysia japonica Steud.) plants regenerated from protoplasts,”Plant Cell Reports, 1998, 17, 334-338.
Park, H.-D., et al., “Construction of binary vectors for the rice transformation using a rice actin promoter and replication origin of pTi12 isolated fromAgrobacterium tumefaciensKU12,”J. of Plant Biol., 1995, 38(4), 365-371.
Park, S.H., et al., “T-DNA integration into genomic DNA of rice followingAgrobacteriuminoculatin of isolated shoot apices,”Plant Molecular Biology, 1996, 32, 1135-1148.
Schledzewski, K., et al., “Quantitative transient gene expression: comparison of the promoters for maize polyubiquitin1, rice actin1, maize-derived Emu and CaMV 35S in cells of barley, maize and tobacco,”Transgenic Res., 1994, 3, 249-255.
Zhong, H., et al., “Transgenic plants of turfgrass (Agrostis palustris Huds) from microprojectile bombardment of embryogenic callus,”Plant Cell Repts., 1993, 13, 1-6.
Lakkaraju Subha R.
Pitcher Lynne H.
Zilinskas Barbara A.
Fox David T.
Rutgers The State University of New Jersey
Woodcock & Washburn LLP
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