Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Via agrobacterium
Reexamination Certificate
2008-02-28
2011-11-15
Hwu, June (Department: 1661)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Via agrobacterium
C800S320100, C435S469000
Reexamination Certificate
active
08058514
ABSTRACT:
A novelAgrobacterium-mediated method for producing a transformed monocotyledonous plant is provided. The transformation method involves (i) a coculture step for culturing anAgrobacterium-inoculated monocotyledonous plant tissue with a coculture medium containing 3,6-dichloro-o-anisic acid, 4-amino-3,5,6-trichloropicolinic acid and/or 2,4,5-trichlorophenoxyacetic acid, and (ii) a regeneration step for culturing the tissue obtained in (i) with a regeneration medium containing a selective drug to thereby induce regeneration and to produce a transformed plant. The method does not include, between the coculture step and the regeneration step, any selection step for culturing the cocultured tissue with a medium containing an auxin and a selective drug to select a transformant.
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Hiei Yukoh
Ishida Yuji
Birch & Stewart Kolasch & Birch, LLP
Hwu June
Japan Tobacco Inc.
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