Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1996-09-06
1998-03-10
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 76, 435125, 435200, 435272, 4241951, 252398, 252404, 252407, 426545, 426546, 426598, 426634, 530370, 530378, 530412, 530414, 549402, 549403, 514 2, 514456, C21P 2106, C12N 924, A61K 31337, A61K 3578
Patent
active
057260346
ABSTRACT:
An aglucone is isoflavone enriched vegetable protein extract and protein material are provided, as well as a high genistein content material and a high daidzein content material. Isoflavone conjugates in a vegetable material are converted to isoflavone glucosides by treating the vegetable material at a temperature and a pH for a period of time sufficient to effect the conversion. The isoflavone glycosides are converted to glucose isoflavones by enzymatic reaction. The vegetable material is extracted with an aqueous extractant having a pH above about the isoelectric point of protein in the vegetable material to extract protein and the isoflavones either before or after conversion of the isoflavone conjugates to isoflavone glucosides or the conversion of the isoflavone glucosides to aglucone isoflavones. An aglucone isoflavone enriched protein material is produced by precipitating the protein and aglucone isoflavones from the extract. A high genistein content material or a high daidzein content material may be produced from the aglucone isoflavone enriched protein extract or aglucone isoflavone enriched protein material by separating the high genistein or high daidzein content material from the extract or protein material.
REFERENCES:
patent: 5320949 (1994-06-01), Shen
patent: 5351384 (1994-10-01), Shen
patent: 5637561 (1997-06-01), Shen et al.
patent: 5637562 (1997-06-01), Shen et al.
Allred Maryann C.
Bryan Barbara A.
Lilling Herbert J.
Protein Technologies International, Inc.
Taylor Richard B.
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