Agglutination immunoassays carried out with agent to reduce non-

Details Agglutination immunoassays carried out with agent to reduce non- Agglutination immunoassays carried out with agent to reduce non-

1981-04-14

1982-12-07

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

23915, 252408, 424 1, 424 12, G01N 3354, G01N 3378

Patent

active

043625312

ABSTRACT:
In particle agglutination immunoassays for an analyte (e.g. an antigen or antibody) in a liquid sample (e.g. human serum), interferences arise due to non-specific protein-protein interactions and the like. These interferences are reduced or overcome by including in the assay mixture a chaotropic or chaotropic-like agent, in a carefully controlled amount. Such agents include guanidine, guanidinium hydrochloride or thiocyanate, sodium or ammonium thiocyanate, and urea, sodium chloride and ethylenediamine tetraacetic acid.

REFERENCES:
patent: 3791932 (1974-02-01), Schuurs
patent: 4200436 (1980-04-01), Mochido
patent: 4292038 (1981-09-01), Kondo
patent: 4298593 (1981-11-01), Ling
patent: 4299815 (1981-11-01), Hansen
patent: 4305925 (1981-12-01), Kapmeyer
H. L. Vader et al., Clinica Chimica Acta, 80, 361-372, (1977).
Chemical Abstracts, 90:20740c, (1979).

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