Agglutination dependent enzyme channeling immunoassay

Details Agglutination dependent enzyme channeling immunoassay Agglutination dependent enzyme channeling immunoassay

1984-02-24

1987-05-05

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435810, 436523, 436533, 436534, 436808, G01N 33546, G01N 33535

Patent

active

046632786

ABSTRACT:
A novel agglutination assay is provided which brings together through the intermediacy of the binding of specific binding pair members particles which differ by having different members of a signal producing system. The signal producing system is characterized by having two enzymes which are related by having the product of one enzyme being the substrate of the other, where the reaction of the second enzyme results in a product providing a detectable signal, desirably including a scavenger for the product of the first enzyme. The amount of product providing the detectable signal which is produced is related to the amount of analyte in the media. The composition and ratio of particles can be provided in reagent kits to optimize the assay results.

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patent: 4299916 (1981-11-01), Litman et al.
patent: 4486530 (1984-12-01), David et al.

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