Agglutination assay

Details Agglutination assay Agglutination assay

1980-04-07

1981-12-15

Turk, Arnold

Chemistry: physical processes

Physical processes

Crystallization

23915, 422 55, 422 61, 424 12, G01N 3354

Patent

active

043057217

ABSTRACT:
An analyte is determined quantitatively by an agglutination assay by use of a method which includes the use of at least two analyte standards having different analyte concentrations; an analyte supported on a particulate support; specific binder for the analyte and buffered diluent for making serial dilutions of an analyte sample. The assay is preferably conducted on a test card which includes a sample portion and a standard portion, with the sample portion having a series of separated marked dilution areas for receiving dilutions of an analyte sample and the standard portion including separated marked standard areas for receiving analyte standard of different analyte concentration. By addition of binder and particles sensitized with analyte to the serial dilutions and standards, analyte can be determined quantitatively by multiplying the lowest standard concentration at which there is no visible agglutination by the highest reciprocal dilution of test sample which similarly shows no visible agglutination.

REFERENCES:
patent: 3424558 (1969-01-01), Eldon
patent: 3502437 (1970-03-01), Mass
patent: 3853468 (1974-12-01), Haymond
patent: 4100268 (1978-07-01), Scherr

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