Agents for the detection of substrates with hydrolase activity

Details Agents for the detection of substrates with hydrolase activity Agents for the detection of substrates with hydrolase activity

1992-08-10

1994-03-08

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 19, 435 21, 548161, 548119, 5483435, 536 173, C12Q 144, C12Q 142, C07D27782, C07D23356

Patent

active

052926699

ABSTRACT:
A method of detecting hydrolase activity using as substrates the compounds of the formula ##STR1## wherein each of R.sup.1, R.sup.2 and R.sup.3 is C.sub.1 -C.sub.4 alkyl, or phenyl optionally substituted in the meta- or para- position by C.sub.1 -C.sub.6 alkyl, C.sub.1 -C.sub.6 alkoxy or mono- or di-(C.sub.1 -C.sub.6)-alkylamino, or optionally substituted by an O-X group in which X is a glycosyl, phosphate or acyl moiety of a natural substrate of the corresponding glycosidase, phosphatase or esterase.

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