Agents and procedures for the transfer of proteins and/or nuclei

Details Agents and procedures for the transfer of proteins and/or nuclei Agents and procedures for the transfer of proteins and/or nuclei

1987-09-02

1989-06-13

Robinson, Ellis P.

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428333, 428515, 4283073, 4283111, 436 86, 525 541, B32B 1710, B32B 2708, G01N 33552, C07K 1700

Patent

active

048392316

ABSTRACT:
The invention relates to a procedure for the transfer and immobilization of proteins onto a free surface formed on a chemically inert support, preferably porous glass, from a medium likely to contain them which is brought into contact with this surface. The said surface is composed of a film of a vinylpyridine polymer in which the nitrogen atom of the pyridyl moiety is substituted by an alkyl group, in particular by a methyl group; the proteins and/or the nucleic acids are brought into contact with the said surface in solution in the presence of a detergent bearing a negative charge, in particular SDS. The invention also relates more particularly to the supports coated with the said films, in particular as a monomolecular layer.

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patent: 4610847 (1986-09-01), Hood et al.
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