Agents and a method of chromosome preparation using protein phos

Details Agents and a method of chromosome preparation using protein phos Agents and a method of chromosome preparation using protein phos

1995-08-14

1998-08-04

Degen, Nancy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435375, 536181, C12Q 100

Patent

active

057891648

ABSTRACT:
Agents and a method for generating chromosomes by premature chromosome condensation (PCC) technique utilizes inhibitors of serine/threonine protein phosphatases. When the cells were treated with these agents, they underwent PCC at any phase of cell cycle within 2 hours. This method enables to obtain not only chromosome of mitotic cells but also those from interphase nuclei much easily, quickly and effectively.

REFERENCES:
Ishida, Y., 1992, Journal of Cellular Physiology, vol. 150, pp. 484-492, 1992.
Chemical Abstracts, vol. 118, No. 25, p. 327, Jun. 21, 1993, AN-249647, A. P. Dyban, et al, "Okadaic Acid Induces Premature Chromosome Condensation Reflecting the Cell Cycle Progression in One-Cell Stage Mouse Embryos", & Mol. Reprod. Dev., vol. 34, No. 4, pp. 402-415, 1993.
Proceedings of the National Academy of Sciences of USA, vol. 89, pp. 10613-10617, Nov. 1992, H. Tosuji, et al., "Calyculin A Induces Contractile Ring-Like Apparatus Formation and Condensation of Chromosomes in Unfertilized Sea Urchin Eggs".
Proceedings of the National Academy of Sciences of USA, vol. 88, pp. 6843-6847, Aug. 1991, K. E. Steinmann, et al., "Chemically Induced Premature Mitosis: Differential Response in Rodent and Human Cells and the Relationship to Cyclin B Synthesis and p34(cdc2)/Cyclin B Complex Formation".
EMBO Journal, vol. 9, No. 13, pp. 4331-4338, 1990, K. Yamashita, et al., "Okadaic Acid, A Potent Inhibitor Type 1 and Type 2A Protein Phosphatases, Activates cdc2/H1 Kinase and Transiently Induces a Premature Mitosis-Like State in BHK21 Cells".
Nature, vol. 226, pp. 717-722, May 23, 1970, R. T. Johnson, et al., "Mammalian Cell Fusion: Induction of Premature Chromosome Condensation in Interphase Nuclei".

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