Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1989-04-13
1992-01-07
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 14, 435 25, 436175, 436129, 436 80, 210759, 252181, C12Q 100, G01N 100, C02F 172, C02F 510
Patent
active
050791400
ABSTRACT:
The present invention is directed to the elimination of ascorbic acid interference in assay systems, particularly assay systems based upon oxidase-peroxidase coupled reactions. When ascorbic acid is present in a sample, it can act as a reductant thereby interfering with an assay's reagent system. The present invention eliminates this inteference by quickly oxidizing any ascorbate thereby preventing ascorbate from acting as an unwanted reductant. The ascorbate is oxidized using a dual oxidant system comprising a water soluble polymer bound to Cu.sup.+2 and an organic or inorganic oxidant such as chromate, peroxide, or a N-halo derivative. This invention is surprisingly selective and generally will not itself interfere with the assay's reagent system. Furthermore, the present invention is so fast and efficient that it can be incorporated into a convenient format, such as a conventional "dip-and-read" reagent test strip system.
REFERENCES:
patent: 4743559 (1988-05-01), Koever et al.
patent: 4910134 (1990-03-01), Yamanishi et al.
patent: 4954451 (1990-09-01), Albarella et al.
patent: 4957872 (1990-09-01), Koever et al.
Albarella James P.
Schick Lloyd A.
Yip Meitak T.
Coe Roger N.
Miles Inc.
Preston David R.
Wax Robert A.
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