Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Conjugate or complex
Reexamination Certificate
2000-02-02
2001-07-10
Bui, Phuong T. (Department: 1761)
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Conjugate or complex
C435S410000, C530S370000, C800S298000, C800S281000, C426S655000, C554S009000
Reexamination Certificate
active
06258361
ABSTRACT:
DESCRIPTION
Technical Field
The present invention relates to an agent for recovering hematopoietic function and a processed food both containing a treated product of peanut seed coats.
Background Art
Antibiotic-type carcinostatics used in chemotherapy for cancer damage the function of bone marrow when administered for a long time, causing hematopoietic dysfunctions (represented by thrombocytopenia) and leukopenia. Among these side effects, the inhibition of lymphocyte functions associated with leukopenia causes remarkable decrease in natural curing ability in humans and infectious diseases in them. Thus, this side effect is a big obstacle to recovery from diseases.
In order to alleviate those side effects occurring in chemotherapy for cancer, recombinant formulations of cytokine genes which activate lymphocyte functions, such as human granulocyte colony-stimulating factor (rhG-CSF), human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) and human macrophage colony-stimulating factor (rhM-CSF), are applied. However, since these medicines are protein formulations prepared by recombinant DNA technology, their cost per day is rather high and yet there are limitations in the term of use and in the administration method (intravenous injection). Therefore, development of effective medicines which are cheap and capable of oral administration is desired.
DISCLOSURE OF THE INVENTION
It is an object of the present invention to provide an agent for recovering hematopoietic function and a processed food both comprising a physiologically active substance which is derived from a natural product and capable of oral administration.
As a result of extensive search for a physiologically active substance having bone marrow cell-proliferating activity which occurs in the natural world and does not cause side effects, the present inventors have found that an extract from peanut seed coats and a treated product of the extract have an effect of proliferating bone marrow cells. Further, it was found that the extract and treated extract exhibit the above-mentioned effect even when administered orally and, thus, they are useful as an agent for recovering hematopoietic function and as a processed food. Thus, the present invention has been achieved.
The present invention includes the following inventions.
(1) An agent for recovering hematopoietic function comprising as an active ingredient a peanut seed coat-derived substance exhibiting bone marrow cell-proliferating activity or a culture obtained by culturing a nonpathogenic fungus in a medium containing peanut seed coats or a treated product of the culture.
(2) An agent for recovering hematopoietic function comprising as an active ingredient an extract from peanut seed coats or a treated product of the extract.
(3) A processed food comprising a peanut seed coat-derived substance exhibiting bone marrow cell-proliferating activity or a culture obtained by culturing a nonpathogenic fungus in a medium containing peanut seed coats or a treated product of the culture.
(4) A processed food comprising an extract from peanut seed coats or a treated product of the extract.
The fruit of peanut has a solid pericarp. Usually, there are two seeds present inside the pericarp. These seeds are covered with seed coats. In the present invention, these seed coats are used.
In the present invention, peanut seed coats are used as an extract or a treated extract, or as a culture obtained by culturing a nonpathogenic fungus in a medium containing peanut seed coats or a treated product of the culture.
In the present invention, the “peanut seed coat-derived substance exhibiting bone marrow cell-proliferating activity” may be any substance as long as it is obtained from peanut seed coat and has activity to proliferate bone marrow cells. For example, an extract from peanut seed coats or the extract which has been given at least one of such treatments as separation, purification, isolation, heating and alkali treatment and which retains activity to proliferate bone marrow cells may be given.
As an extraction solvent, water; alcohols such as methanol, ethanol, propanol, butanol; ethers such as ethyl ether, dioxane; ketones such as acetone; or the like may be used. When an extract is used as a dry material after removal of the solvent, any of the above-mentioned solvents may be used alone or in combination. When an extract is used in a state of solution dissolved in the solvent, a solvent harmless to human body must be used. In this case, it is preferable to use water, ethanol or a mixture of water and ethanol.
In the extraction, peanut seed coats may be used without any treatment. Alternatively, they may be crushed or powdered to give greater contact with the solvent.
A waste water generated in the process of separating peanut seed coats from peanut seeds by washing them with water and then purified or dried if necessary may be used as an extract. However, it is preferable to extract peanut seed coats with a solvent in an amount of 5 to 25 liters per kg of the coats.
Extraction temperature ranges preferably from room temperature to the boiling point of the solvent under ambient pressure. Extraction period is preferably one day though it varies depending on the extraction temperature.
The thus obtained extract as it is may be used as a component of the agent for recovering hematopoietic function or the processed food of the invention. Alternatively, the extract may be treated by various means of purification such as ion exchange chromatography, gel filtration chromatography, dialysis, etc. to obtain active fractions using bone marrow cell-proliferating activity as an indicator. Then, such a treated extract with enhanced activity may be used in the invention.
In the present invention, instead of the above-described extract or treated extract, a culture obtained by culturing a nonpathogenic fungus in a medium containing peanut seed coats or a treated product of the culture may be used. The culture or treated culture is especially preferable for use in various processed foods including health food.
The nonpathogenic fungus used in the invention is not particularly limited. For example, basidiomycetes such as polypores,
Tricholoma matsutake
(matsutake fungus),
Lentinus endodes
(shiitake fungus),
Grifola frondosa
; and ascomycetes such as
Onygena corvina
and fungi belonging to the genus Cordyceps may be enumerated.
The above-described culture obtained by culturing a nonpathogenic fungus in a medium containing peanut seed coats may be used as it is as a component of the agent for recovering hematopoietic function or the processed food of the invention. Alternatively, the culture may be given a physicochemical treatment such as such photo treatment or pH adjustment; or a biochemical treatment such as fermentation and used as a treated culture.
The agent of the invention for recovering hematopoietic function can be formulated into pharmaceutical preparations by using an extract from peanut seed coats or a treated product of the extract, or a culture obtained by culturing a nonpathogenic fungus in a medium containing peanut seed coats or a treated product of the culture alone or in combination with a pharmaceutical carrier. The dosage form is not particularly limited and may be appropriately selected according to the route and method of administration. Generally, the agent of the invention is used as oral agents such as tablets, capsules, granules, fine granules or powder.
The dosage of the agent of the invention for recovering hematopoietic function varies depending on the age, weight and degree of the disease of a patient. In oral administration, the dosage ranges usually from 100 to 500 mg per day as dry powder of peanut seed coat extract. Usually, the number of administration is once to three times a day in oral administration.
In the present invention, a culture obtained by culturing a nonpathogenic fungus in a medium containing peanut seed coats or a treated product of the culture is preferably used as a component of processed foods. The dosage in this case is usually
Fushiya Shinji
Hojo Hiroshi
Takano Fumihide
Yahagi Nobuo
Yoshihara Akio
Bui Phuong T.
Pennie & Edmonds LLP
Yoshihara Akio
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