Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-10-02
2007-10-02
Strzelecka, Teresa E. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200
Reexamination Certificate
active
11232313
ABSTRACT:
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays, systems, methods and kits for the age determination of an individual from bloodstains or samples of unknown origin. The methodology is based on gene expression profiling analysis in which novel human newborn fetal specific genes are identified by detecting the presence of appropriate messenger RNA species.
REFERENCES:
Chomczynski, P. et al., “Single-step method of RNA isolation by acid guanidinium thiocyanate- phenol-choloform extraction.” (1987),Analytical Biochemistry, pp. 162, 156-159.
Juusola, J. and Ballantyne, J., “Messenger RNA profiling: a prototype method to supplant conventional methods for body fluid identification.” (2003),Forensic Science International 135pp. 85-96.
Livak, J. and Schmittgen, T., “Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCTMethod.” (2001),Methods 25, pp. 402-408.
Alvarez Michelle
Ballantyne John
Calamita Heather
Law Offices of Brian S.Steinberger, P.A.
Morlin Joyce P.
Steinberger Brian S.
Strzelecka Teresa E.
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