Agarose plug mold and processing chamber

Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Treating animal or plant material or micro-organism

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

935 85, 425 89, 249141, 249160, 249168, C12J 320, B28B 1500, B29C 3346, B41B 1154

Patent

active

054570506

ABSTRACT:
An apparatus and method for the preparation of large DNA molecules from cells which have been cast in agarose. Specifically, the invention provides for a processing chamber that allows agarose plugs to be molded and processed within the same apparatus. This greatly reduces the amount of manipulation required of such DNA samples and reduces the loss of material due to agarose plug breakage. The mold has a filling port for agarose and a slot for at least one retainer for preventing the molten agarose from exiting through openings in the mold through which DNA processing solutions later access the molded agarose plugs.

REFERENCES:
patent: 4944483 (1990-07-01), Nishizawa
Arbeit et al., "Resolution of Recent Evolutionary Divergence among Escherichia coli from Related Lineages: The Application of Pulsed Field Electrophoresis to Molecular Epidemiology," The Journal of Infectious Diseases, 161:230-235, 1990.
Hector et al., "Large Restriction Fragment Patterns of Genomic Mycobacterium fortuitum DNA as Strain-Specific Markers and Their Use in Epidemiologic Investigation of Four Nosocomial Outbreaks," Journal of Clinical Microbiology, 30(5):1250-1255 (Aug., 1992).
Mazurek et al., "Large DNA Restriction Fragment Polymorphism in the Mycobacterium avium-M, intracellulare Complex: a Potential Epidemiologic Tool," Journal of Clinical Microbiology, 31(2):390-394, 1993.
Patterson et al., "Epidemiology of an Endemic Strain of S-Lactamase-Producing Enterococcus faecalis," Journal of Clinical Microbiology, 29(11):2513-2516, 1991.
Zhang et al., "DNA Polymorphisms in Strains of Mycobacterium tuberculosis Analyzed by Pulsed-Field Gel Electrophoresis: a Tool for Epidemiology," Journal of Clinical Microbiology, 30(6):1551-1556, 1992.
Birren and Lai, "Pulsed Field Gel Electrophoresis: A Practical Guide," Academic Press, Inc., 1993, pp. 27-31.
Schwartz, D. and Cantor, C. "Separation of Yeast Chromosomes Sized DNA by Pulsed Field Gradient Gel Electrophoresis", Cell vol. 37 pp. 67-75 (1984).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Agarose plug mold and processing chamber does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Agarose plug mold and processing chamber, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Agarose plug mold and processing chamber will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2310049

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.