Agarose gel electrophoresis technique for the determination of a

Details Agarose gel electrophoresis technique for the determination of a Agarose gel electrophoresis technique for the determination of a

1983-03-23

1985-12-17

Metz, Andrew H.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

201299R, 2523153, 435 22, 436806, G01N 2726, B01D 1508

Patent

active

045591202

ABSTRACT:
Separation of all clinically relevant isoamylase bands including P.sub.1 P.sub.1b, P.sub.2, P.sub.3, P.sub.4, S.sub.1, S.sub.2, S.sub.3, S.sub.4 and S.sub.5 is achieved in a test in which a biological sample is electrophoresed on an agarose gel supporting a tris-sodium barbital-barbital buffer at a pH of between 8.4 and 9.2. The barbital anion concentration is between about 0.03 and about 0.08 M, the tris cation concentration is between about 0.03 M and about 0.07 M; and the sodium cation concentration is between about 0.03 M and about 0.07 M. Agarose is present in the gel at concentrations of between about 0.4 and about 1.5 weight percent.

REFERENCES:
patent: 3497437 (1970-02-01), Louderback et al.
patent: 3527712 (1970-09-01), Renn et al.
patent: 3687833 (1972-08-01), Parcells et al.
patent: 3912610 (1975-10-01), Lou
patent: 3998696 (1976-12-01), Yomoto et al.
patent: 4030995 (1977-06-01), Starkweather
patent: 4209372 (1980-06-01), Bluestein et al.
patent: 4246084 (1981-01-01), Gurske
patent: 4454161 (1984-06-01), Okada et al.
Pierre Leclerc and Jean-Claude Forest, Clinical Chemistry, vol. 28, No. 1, 1982.

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