Agarase and gene thereof

Details Agarase and gene thereof Agarase and gene thereof

2007-11-30

2009-11-24

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S208000, C435S252300, C435S320100, C435S084000, C536S023200

Reexamination Certificate

active

07622291

ABSTRACT:
Production of efficiently producing agaro-oligosaccharide; a polypeptide having an agarase activity which is usable in, for example, efficiently extracting a substance such as a nucleic acid from an agarose gel; the amino acid sequence of the polypeptide; a gene encoding the polypeptide; a process for producing the polypeptide; a process for producing agaro-oligosaccharide; and a process for extracting a substance such as a nucleic acid from an agarose gel.

REFERENCES:
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Kwan, S., at al.,Enzymic Cleavage of the α-Linkages in Agarose, to Yield Agaro-Oligosaccharides. Carbohydrate Research, vol. 66 (1978), pp. 207-212.
Potin. P., et al.,Purification and Characterization of the α-agarase from Altermonas agarlyticus(Cataldi)comb. nov., strain GJ1B. European Journal of Biochemistry, No. 214 (1993), pp. 599-607.
Database EMBL ′Online!, Jan. 21, 2000, retrieved from EMBL, Database accession No. AF121273 XP002271973 Sequence of alpha-agarase fromAlteromonas agarilytica.
Database EMBL Online, Database Accession No. M73783 XP 002271971, Sequence of Beta-Agarase I Gene from Pseudomonas Atlantica, Mar. 12, 1996.
Database EMBL Online, Database Accession No. U61972 XP 002271972, Sequence of Beta-Agarase Gene from Aeromonas sp., Oct. 20, 1999.
Vera, et al., “Identification of a Marine Agarolytic Pseudoalteromonas Isolate and Characterization of Its Extracellular Agarase”, Applied and Environmental Microbiology. Nov. 1998, vol. 64, No. 11, 4378-4383.
Hassairi at al.,Bioresource Tech., 79 :47-51 (2001).

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